YILIMART - CD11c MicroBeads UltraPure, mouse

In mice CD11c is a well-established, yet not exclusive, pan-DC marker. CD11c MicroBeads UltraPure have been optimized for the isolation of mouse DCs from single-cell suspensions from lymphoid and non-lymphoid tissues. The CD11c MicroBeads UltraPure allow for the rapid and simple isolation of all defined murine DC subsets. The UltraPure beads greatly improve recovery and purity of the sorted population by specifically enriching viable cells.

Background information

The mouse CD11c antigen is present in dendritic cells in lymphoid organs and blood, in Langerhans cells in the epidermis, in dendritic cell progenitors in the bone marrow, and in in vitro generated bone marrow–derived dendritic cells. In mouse spleen and lymph node, CD11c is expressed at high levels on conventional CD11c

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CD45R

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mPDCA-1

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dendritic cells, and at moderate levels on CD11c

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CD45R

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mPDCA-1

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plasmacytoid dendritic cells. CD11c is reported to be weakly expressed on NK cells, B cells, and T cell subsets. About 1–3% of splenocytes, 2% of bone marrow cells, as well as <1% of lymph node cells and thymocytes express CD11c.

Downstream applications

DCs isolated with CD11c MicroBeads have been used for phenotypical and functional analyses, e.g. T cell activation, polarization, and tolerance induction in different experimental murine models. CD11c MicroBeads were used to isolate highly pure DCs from spleen and lymph nodes

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, Peyer's patches

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, colonic lamina propria

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, bone marrow

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, epidermis

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, lung

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, liver

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, or brain

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Columns

For positive selection: MS, LS, XS, or autoMACS

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Columns. For depletion: LD, D, or autoMACS Columns.

Figure 1

CD11c

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DCs were isolated from mouse spleen using CD11c MicroBeads UltraPure, two MS Columns, and a MiniMACS™ Separator. Cells were fluorescently stained with CD11c-VioBlue (# 130‑102‑413) and analyzed by flow cytometry using the MACSQuant

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Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Before separation	CD11c ⁺ dendritic cells

Selected references
1. Williamson, E. et al. (2002) Regulation of mucosal dendritic cell function by receptor activator of NF-κB (Rank)/Rank ligand interactions: impact on tolerance induction. J. Immunol. 169: 3606-3612
2. Ing, R. et al. (2006) Interaction of mouse dendritic cells and malaria-infected erythrocytes: uptake, maturation, and antigen presentation. J. Immunol. 176: 441-450
3. Kadaoui. K. A. and Corthésy, B. et al. (2004) Isolation of dendritic cells from mouse Peyer’s patches using magnetic cell sorting. MACS&more 8(1): 10-12
4. Krajina, T. et al. (2003)
  
  Colonic lamina propria dendritic cells in mice with CD4
  ⁺
  T cell-induced colitis.
  
  Eur. J. Immunol. 33: 1073-1083
5. Hou, W. S. and van Parijs, L. et al. (2004) A Bcl-2-dependent molecular timer regulates the lifespan and immunogenicity of dendritic cells. Nat. Immunol. 5: 583-589
6. Sato, T. et al. (2002) Consequences of OX40-OX40 ligand interactions in langerhans cell function: enhanced contact hypersensitivity responses in OX40L-transgenic mice. Eur. J. Immunol. 32: 3326-3335
7. Swanson, K. A. et al. (2004) Flt3-Ligand, Il-4, GM-CSF, and adherencemediated isolation of murine lung dendritic cells: assessment of isolation technique on phenotype and function. J. Immunol. 173: 4875-4881
8. Johansson, C. and Wick, M. J. (2004)
  
  Liver dendritic cells present bacterial antigens and produce cytokines upon
  Salmonella
  encounter.
  
  J. Immunol. 172: 2496-2503
9. Fischer, H. G. et al. (2000)
  
  Phenotype and functions of brain dendritic cells emerging during chronic infection of mice with
  Toxoplasma gondii
  .
  
  J. Immunol. 164: 4826-4834
Brochures and posters

MACS Cell Pre-Enrichment for flow sorting

Dendritic cells - Quick guide

Human and mouse DC generation
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Mouse cross-presenting DC

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CD11c MicroBeads UltraPure, mouse

For research use only

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Background information

Downstream applications

Columns

Figure 1

Selected references

Brochures and posters

Scientific posters

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