YILIMART - CD8 MicroBeads, human

CD8 MicroBeads were developed for the positive selection or depletion of human cytotoxic CD8

⁺

T cells from different cell sources. The most commonly used cell sources are peripheral blood and cord blood. In addition, cytotoxic T cells have also been isolated from lymph nodes, thymus, skin biopsies

¹

, and spleen.

Background information

The CD8 antigen forms a complex together with the T cell receptor and acts as an accessory molecule in the recognition of MHC class I/peptide complexes by the TCR heterodimer on CD8

⁺

cytotoxic T cells. The CD8 molecule consists of either an α/β heterodimer or an α/α homodimer. It is expressed strongly on cytotoxic T cells and dimly on a subset of NK cells. CD8 is found on most thymocytes and on about one third of all peripheral blood T cells. CD8

⁺

cytotoxic T cells play an important role in the killing of virus-infected cells and tumor cells.

Downstream applications

Isolation or depletion of CD8

⁺

cytotoxic T cells is performed in many different research fields such as infectious diseases, autoimmune diseases, allergy, and asthma, as well as tumor immunology.

Cytotoxic T cells isolated by MACS® Technology remain viable and functional. Therefore, they can be used for further functional studies, such as proliferation assays

^2,9

and cytotoxicity assays

^2,3

, but also for the analysis of

in vitro

cytokine production

⁴

. CD8 MicroBeads have also been used for the depletion of CD8

⁺

T cells from human PBMCs for immune reconstitution experiments in SCID mice.

⁵

In combination with CD4 MultiSort MicroBeads, CD8 MicroBeads have been used for the isolation of CD4

⁺

CD8

⁺

double-positive thymocytes.

⁸

Other examples include various studies on viral infections, e.g.,

in vitro

infections of CD8

⁺

cells with HIV

⁶

, or monitoring of immune abnormalities in children of HIV-infected mothers

⁷

.

Columns

For positive selection: MS, LS, XS, or autoMACS

^®

Columns. For depletion: LD, D, or autoMACS Columns.

Figure 1

Separation of CD8

⁺

cells from PBMCs using CD8 MicroBeads, an LS Column, and a MidiMACS™ Separator.

PBMCs before separation	CD8 ^- cells

CD8 ⁺ cells

Figure 2
View details

Figure 2

Scanning electron micrograph of CD8
⁺
T cells isolated using CD8 MicroBeads (courtesy of Prof. Peter Groscurth, Institute of Anatomy, University of Zürich, Switzerland).

Scanning electron micrograph of CD8
⁺
T cells isolated using CD8 MicroBeads (courtesy of Prof. Peter Groscurth, Institute of Anatomy, University of Zürich, Switzerland).

Selected references
1. Akdis, M. et al. (1999)
  
  Skin homing (cutaneous lymphocyte-associated antigen-positive) CD8
  ⁺
  T cells respond to superantigen and contribute to eosinophilia and IgE production in atopic dermatitis.
  
  J. Immunol. 163: 466-475
2. Parra, E. et al. (1997)
  
  The role of B7-1 and LFA-3 in costimulation of CD8
  ⁺
  T cells.
  
  J. Immunol. 158: 637-642
3. Turner, J. and Dockrell, H M. (1996)
  
  Stimulation of human peripheral blood mononuclear cells with live
  Mycobacterium bovis
  BCG activates cytolytic CD8
  ⁺
  T cells
  in vitro
  .
  
  Immunology 87: 339-342
4. Akdis, A. C. et al. (1995) Cytokine immunotrapping: an assay to study the kinetics of production and consumption or degradation of human interferon-gamma. J. Immunol. Methods 182: 251-261
5. Walker, W. and Gallagher, G. (1994)
  
  The
  in vivo
  production of specific human antibodies by vaccination of human-PBL-SCID mice.
  
  Immunology 83: 163-170
6. Ennen, J. et al. (1994) CD8+ T lymphocytes of African green monkeys secrete an immunodeficiency virus-suppressing lymphokine. Proc. Natl. Acad. Sci. U.S.A. 91: 7207-7211
7. Nielsen, S. D. et al. (2001) Impaired progenitor cell function in HIV-negative infants of HIV-positive mothers results in decreased thymic output and low CD4 counts. Blood 98: 398-404
8. Kutlesa, S. et al. (2002) Developmentally regulated interactions of human thymocytes with different laminin isoforms. Immunology 105: 407-418
9. Al-Shanti, N. et al. (2004)
  
  Investigation of alpha nascent polypeptide-associated complex functions in a human CD8
  ⁺
  T cell
  ex vivo
  expansion model using antisense oligonucleotides.
  
  Immunology 112: 397-403
Brochures and posters

MACS Cell Pre-Enrichment for flow sorting
Customer reports and application notes

Generation of functional CAR T cellsfrom CD4
⁺
and CD8
⁺
T cells

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CD8 MicroBeads, human

For research use only

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Background information

Downstream applications

Columns

Figure 1

Figure 2

Figure 2

Selected references

Brochures and posters

Customer reports and application notes

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