YILIMART - CD4 MicroBeads, human

CD4 MicroBeads were developed for the positive selection or depletion of CD4

⁺

cells by direct magnetic labeling. The most commonly used cell sources are peripheral blood, leukapheresis

¹

, and cord blood. In addition, T helper cells have also been isolated from lymph nodes, thymus, spleen, skin biopsies

²

, synovial fluids, and cell cultures.

Background information

CD4 is an accessory molecule in the recognition of MHC class II/peptide complexes by the TCR heterodimers on CD4

⁺

T helper cells. CD4 is expressed on T helper cells and at a lower level on monocytes and dendritic cells. The CD4 molecule is the receptor for the human immunodeficiency virus. The CD4 antibody recognizes most thymocytes and about 65% of all peripheral blood T cells.

Downstream applications

Isolation or depletion of CD4

⁺

T helper cells is performed in many different fields of research, such as infectious diseases, autoimmune diseases, allergy, asthma, and tumor immunology. T helper cells isolated by MACS® Technology remain viable and functional. Therefore, they can be used for further functional studies, such as proliferation assays

¹⁰

and cytotoxicity assays

³

, as well as for the generation of T cell lines

⁹

. CD4

⁺

T cells purified with CD4 MicroBeads have also been cultured and analyzed for

in vitro

cytokine production.

⁴

Furthermore, they have been used in co‑culture experiments, e.g., for allogeneic stimulation by dendritic cells

¹

or for B cell activation

²

.

T helper cells isolated by MACS

^®

Technology were also used for studies on viral infections, e.g., for

in vitro

infections of cells with HIV

⁵

, to determine the infectivity of HIV-infected follicular dendritic cells

⁶

, immune reconstitution after anti-viral therapy

⁷

, and for monitoring of immune abnormalities in children of HIV-infected mothers

⁸

.

Columns

For positive selection: MS, LS, XS, or autoMACS

^®

Columns. For depletion: LD, D, or autoMACS Columns.

Figure 1
View details

Figure 1

Isolation of CD4
⁺
cells from PBMCs using CD4 MicroBeads, an MS Column, and a MiniMACS™ Separator.

Isolation of CD4
⁺
cells from PBMCs using CD4 MicroBeads, an MS Column, and a MiniMACS™ Separator.
Figure 2
View details

Figure 2

CD4
⁺
T helper cells isolated from PBMCs using CD4 MicroBeads. Cells were stained with CD4-FITC in parallel to magnetic labeling.

CD4
⁺
T helper cells isolated from PBMCs using CD4 MicroBeads. Cells were stained with CD4-FITC in parallel to magnetic labeling.

Selected references
1. Jonuleit, H. et al. (2000)
  
  Induction of interleukin 10-producing, nonproliferating CD4
  ⁺
  T cells with regulatory properties by repetitive stimulation with allogeneic immature human dendritic cells.
  
  J. Exp. Med. 192: 1213-1222
2. Akdis, M. et al. (1999)
  
  Skin homing (cutaneous lymphocyte-associated antigen-positive) CD8
  ⁺
  T cells respond to superantigen and contribute to eosinophilia and IgE production in atopic dermatitis.
  
  J. Immunol. 163: 466-475
3. Parra, E. et al. (1997)
  
  The role of B7-1 and LFA-3 in costimulation of CD8
  ⁺
  T cells.
  
  J. Immunol. 158: 637-642
4. Akdis, A. C. et al. (1995) Cytokine immunotrapping: an assay to study the kinetics of production and consumption or degradation of human interferon-gamma. J. Immunol. Methods 182: 251-261
5. Ennen, J. et al. (1994) CD8+ T lymphocytes of African green monkeys secrete an immunodeficiency virus-suppressing lymphokine. Proc. Natl. Acad. Sci. U.S.A. 91: 7207-7211
6. Heath, S. L. et al. (1995) Follicular dendritic cells and human immunodeficiency virus infectivity. Nature 377: 740-744
7. Douek, D. C. et al. (1998) Changes in thymic function with age and during the treatment of HIV infection. Nature 396: 690-695
8. Nielsen, S. D. et al. (2001) Impaired progenitor cell function in HIV-negative infants of HIV-positive mothers results in decreased thymic output and low CD4 counts. Blood 98: 398-404
9. Kim, J. Y. et al. (2004) BH3-only protein Noxa is a mediator of hypoxic cell death induced by hypoxia-inducible factor 1alpha. J. Exp. Med. 199: 113-1142
10. Engedal, N. et al. (2006) All-trans retinoic acid stimulates IL-2-mediated proliferation of human T lymphocytes: early induction of cyclin D3. J. Immunol. 177: 2851-2861
Brochures and posters

MACS Cell Pre-Enrichment for flow sorting
Customer reports and application notes

Generation of functional CAR T cellsfrom CD4
⁺
and CD8
⁺
T cells

Vasoactive intestinal peptide inhibits IFN-α secretion and modulates the immune function of plasmacytoid dendritic cells. Fabricius et al. (2010) MACS&more 12(1).
Special protocols

Isolation of CD4
⁺
T helper cells from human PBMCs

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CD4 MicroBeads, human

For research use only

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Background information

Downstream applications

Columns

Figure 1

Figure 1

Figure 2

Figure 2

Selected references

Brochures and posters

Customer reports and application notes

Special protocols

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