YILIMART - CD33 MicroBeads, human

CD33 MicroBeads have been developed for separation of cells based on the expression of the CD33 antigen. CD33

⁺

cells can be enriched from PBMCs, bone marrow¹

^,

², or tissues.³

Background information

The CD33 antigen is brightly expressed on monocytes and dimly on granulocytes and dendritic cells. The CD33 antigen is also found on myeloid progenitor cells but is not expressed on lymphocytes, platelets, erythrocytes, and primitive hematopoietic stem cells.

Applications

CD33

⁺

cells can be enriched from PBMCs, bone marrow

^1,2

, or tissues.

³

Additionally, the CD33 antigen can be used to distinguish acute myeloid leukemias from lymphoblastic leukemias.

Columns

For positive selection: MS, LS, XS, or autoMACS

^®

Columns. For depletion: LD, D, or autoMACS Columns.

Figure 1

Separation of CD33

⁺

cells from human PBMCs using CD33 MicroBeads, a MiniMACS™ Separator, and an MS Column. Cells are fluorescently stained with CD33-PE and CD14-APC.

PBMCs before separation	Isolated CD33 ⁺ cells

Selected references
1. Czerniecki, B. J. et al. (1997) Calcium ionophore-treated peripheral blood monocytes and dendritic cells rapidly display characteristics of activated dendritic cells. J. Immunol. 159: 3823-3837
2. Gonzalez, R. et al. (1999) Transduction of bone marrow cells by the AdZ.F(pK7) modified adenovirus demonstrates preferential gene transfer in myeloma cells. Hum. Gene Ther. 10: 2709-2717
3. Rogler et al. (1998) Isolation and phenotypic characterization of colonic macrophages. Clin. Exp. Immunol. 112: 205-215

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CD33 MicroBeads, human

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Background information

Applications

Columns

Figure 1

Selected references

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