Background information

Applications

Columns

• Figure 1

  Separation of human leukocyte-enriched buffy coat using CD45 MicroBeads, an MS Column, and a MiniMACS™ Separator. Cells are stained with CD45-FITC.

  Peripheral blood before separation	CD45 – cells
  CD45 + cells

• Figure 2

  Enrichment of tumor cells from a mixture of PBMCs and cells of the breast cancer cell line SK-BR3 using CD45 MicroBeads. (A) Cell mixture before separation (7×106 PBMCs were spiked with 20,000 tumor cells) (B) Negative fraction with enriched breast cancer cells

  A):	B):
  Cell mixture before separation (7×10 6 PBMCs were spiked with 20,000 tumor cells)

• Figure 3

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  Figure 3

  A cytokeratin-positive cell was enriched from bone marrow of a patient with renal carcinoma by depletion of leukocytes using CD45 MicroBeads. Cells were stained using a conventional APAAP method followed by staining with hematoxylin. (Courtesy of Dr. Bilkenroth, Dept. Pathol. and Dr. Meye, Dept. Urol., Univ. Halle, Germany.)

  
  A cytokeratin-positive cell was enriched from bone marrow of a patient with renal carcinoma by depletion of leukocytes using CD45 MicroBeads. Cells were stained using a conventional APAAP method followed by staining with hematoxylin. (Courtesy of Dr. Bilkenroth, Dept. Pathol. and Dr. Meye, Dept. Urol., Univ. Halle, Germany.)

• Selected references

  1. Meye, A. et al. (2002) Isolation and enrichment of urologic tumor cells in blood samples by a semi-automated CD45 depletion autoMACS protocol. Int. J. Oncol. 21: 521-530
  2. Jiang, Y. et al. (2002) Pluripotency of mesenchymal stem cells derived from adult marrow. Nature 418: 41-49

• Brochures and posters

  MACS Cell Pre-Enrichment for flow sorting