YILIMART - CD163 MicroBead Kit, human

The CD163 MicroBead Kit, human has been developed for the enrichment of human cells based on the expression of the CD163 antigen. CD163 is most abundantly expressed by mature tissue macrophages and peripheral blood monocytes. The expression of CD163 is up-regulated

in vitro

and

in vivo

by anti-inflammatory mediators. Depending on the ligand, crosslinking of CD163 initiates signal transduction leading to the production of proinflammatory cytokines, such as IL-1ß, IL-6, and GM-CSF or the anti-inflammatory cytokine IL-10.

Background information

CD163 is most abundantly expressed by mature tissue macrophages and peripheral blood monocytes. CD163 expression is up-regulated

in vitro

and

in vivo

by anti-inflammatory mediators.Depending on the ligand, crosslinking of CD163 initiates signal transduction leading to the production of pro-inflammatory cytokines, such as IL-1ß, IL-6, and GM-CSF or the anti-inflammatory cytokine IL-10.

Detailed separation procedure

The CD163

⁺

cells are indirectly magnetically labeled with CD163-Biotin and Anti-Biotin MicroBeads. The magnetically labeled CD163

⁺

cells are retained in a MACS® Column in the magnetic field of a MACS Separator, while the unlabeled cells pass through the column. The CD163

⁺

cells can then be eluted by removing the column from the magentic field.

Downstream applications

Positive selection of human cells expressing the CD163 antigen
Isolation or depletion of CD163⁺ cells from human peripheral mononuclear cells or single-cell suspensions from, e.g. tumor tissues
Enrichment of CD163⁺ macrophages from single-cells suspension of human tumor samples

Columns

MS, LS, or autoMACS

^®

Columns.

Figure 1

CD163

⁺

cells were isolated from human peripheral blood mononuclear cells (PBMCs) (A) or ovarian carcinoma samples (B) using the CD163 MicroBead Kit, an MS Column, and a MiniMACS™ Separator. Cells were fluorescently stained with CD45-VioBlue

^®

as well as with CD14-FITC and CD163-VioBlue (A) or CD206-FITC and CD163-PE (B) and analyzed by flow cytometry using the MACSQuant

^®

Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

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CD163 MicroBead Kit, human

For research use only

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Background information

Detailed separation procedure

Downstream applications

Columns

Figure 1

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