Background information

Downstream applications

Columns

• Figure 1

  Human carcinoma cells were purified from a mixture of dissociated FFPE tumor cells spiked in dissociated FFPE spleen cells using Anti-Cytokeratin MicroBeads, human. Isolation was performed on an autoMACS ® Pro Separator. The cells were fluorescently stained with Anti-Cytokeratin-FITC, Anti-Vimentin-APC, and CD235a (Glycophorin A)-PE, and DAPI and analyzed by flow cytometry using the MACSQuant ® Analyzer. Aggregates were excluded from the analysis based on scatter signals and single cells were gated on DAPI fluorescence.

  Before separation	After separation

• Selected references

  1. Heatley, M. et al. (1995) Cytokeratin intermediate filament expression in benign and malignant breast disease. J. Clin. Pathol. 48: 26-32

• Customer reports and application notes

  Enrichment of tumor cells from FFPE lung adenocarcinoma resection specimens increases sensitivity of mutation analysis. Wölwer, C. et al. (2020)