YILIMART - CD90.1 MicroBeads, mouse and rat

CD90.1 MicroBeads, mouse and rat, were developed for positive selection or depletion of mouse or rat T cells from single-cell suspensions of lymphoid and non-lymphoid tissues or from peripheral blood.

Background information

The mouse monoclonal antibody reacts with rat CD90 (Thy-1) and mouse CD90.1 (Thy1.1), a GPI-anchored conserved membrane glycoprotein. In the rat, the CD90 antigen is expressed on thymocytes

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, recent thymic emigrants

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, hematopoietic stem cells, neurons, and other cell types. In the mouse strains AKR/J, PL, and FVB/N, CD90.1 is a pan T cell marker and can be found on thymocytes, hematopoietic stem cells in the bone marrow, intraepithelial cells (dendritic epidermal cells) in skin, and on neurons, such as retinal ganglion cells. The antibody does not cross-react with CD90.2 (Thy1.2).

Downstream applications

CD90.1 MicroBeads are suitable for positive selection or depletion of mouse (strains AKR/J, PL, and FVB/N) or rat T lymphocytes from single-cell suspensions of spleen preparations or peripheral blood.
Isolation of T cells for the adoptive transfer of CD90.1⁺ T cells into CD90.2⁺ inbred strains or immunodeficient mice.

Columns

For positive selection: MS, LS, or autoMACS

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Columns. For depletion: LD or autoMACS Columns.

Figure 1

Single-cell suspensions from mouse or rat spleen were prepared using the gentleMACS™ Dissociator. CD90.1

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T cells were isolated from the single-cell suspensions using the CD90.1 MicroBeads, an autoMACS

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Column, and autoMACS Pro Separator. Cells were fluorescently stained with REAfinity™ Antibodies. Mouse cells were stained with CD45-FITC, CD45R(220)-PE-Vio

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770 and CD90.1-VioBlue

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(A, B) and rat cells were stained with CD90.1-VioBlue (C, D). Cells were analyzed by flow cytometry using the MACSQuant

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Analyzer. Viable leukocytes were gated for analysis based on scatter signals, 7-AAD Staining Solution fluorescence, and CD45 expression.