Background information

• Figure 1

  Siglec-F + eosinophils were isolated from mouse peripheral blood using Anti-Siglec-F MicroBeads, two MS Columns, and a MiniMACS™ Separator. Cells were fluorescently stained with Anti-Siglec-F-APC and Anti-Gr-1-VioGreen™ and analyzed by flow cytometry using the MACSQuant ® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

  Before separation	Siglec-F + cells

• Figure 2

  Siglec-F + alveolar macrophages and eosinophils were isolated from mouse lung single-cell suspension using Anti-Siglec-F MicroBeads, two MS Columns, and a MiniMACS Separator. Cells were fluorescently stained with Anti-Siglec-F-APC and CD11c-PE‑Vio ® 770 and analyzed by flow cytometry using the MACSQuant Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

  Before separation	Siglec-F + cells

• Selected references

  1. Zhang, M. et al. (2007)
     Defining the

     in vivo

     function of Siglec-F, a CD33-related Siglec expressed on mouse eosinophils.
     Blood 109(10): 4280-4287
  2. Guo, J. P. et al. (2011) Characterization of expression of glycan ligands for Siglec-F in normal mouse lungs. Am. J. Respir. Cell Mol. Biol. 44(2): 238-243
  3. Bochner, B. (2009) Siglec-8 on human eosinophils and mast cells, and Siglec-Fon murine eosinophils, are functionally related inhibitory receptors. Clin. Exp. Allergy 39(3): 317-324