Background information

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• Figure 1

  A tumor induced by the B16-OVA cell line was dissociated using the gentleMACS™ Octo Dissociator with Heaters in combination with the Tumor Dissociation Kit, mouse. CD4 + and CD8 + TILs were isolated from the single-cell suspension using CD4/8 (TIL) MicroBeads, two MS Columns, and a MiniMACS™ Separator. Cells were fluorescently stained with CD4-PE-Vio ® 615 and CD8b-VioBlue ® and analyzed by flow cytometry using the MACSQuant ® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and Viobility™ 405/520 Fixable Dye fluorescence.

  Before separation	CD4 + and CD8 + cells

• Scientific posters

  Optimized workflow improves the characterization of tumor-infiltrating T cells. SITC (2017)