YILIMART - NK Cell Isolation Kit, mouse

The NK Cell Isolation Kit was developed for the isolation of untouched NK cells from single-cell suspensions of murine spleen. Non-NK cells, i.e. T cells, dendritic cells, B cells, granulocytes, macrophages, and erythroid cells are magnetically labeled by using a cocktail of biotin-conjugated antibodies and Anti-Biotin MicroBeads. Isolation of highly pure unlabeled NK cells is achieved by depletion of non-target cells. The kit has been optimized to give outstanding purities in C57BL/6J mice.

Downstream applications

NK cells isolated using the NK Cell Isolation Kit can be used for

in vitro

and

in vivo

applications, e.g., functional studies or adoptive transfer experiments.

Columns

MS, LS, or autoMACS

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Columns.

Figure 1

NK cells were isolated from a BALB/c (A) or C57BL/6 (B) mouse spleen cell suspension by using the NK Cell Isolation Kit, an LS Column, and a MidiMACS™ Separator. The cells were fluorescently stained with CD3-FITC, CD49b-APC, and Anti-NKp46-PE (A), or CD3-FITC, CD49b-APC, and Anti-NK1.1-PE (B) and analyzed by flow cytometry using the MACSQuant

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Analyzer 10. Cell debris and dead cells were excluded from the analysis based on scatter signals, CD45-VioGreen™, and 4’,6-diamidino-2-phenylindole(DAPI) fluorescence.

A) BALB/c mouse spleen cell suspension