YILIMART - Neuron Isolation Kit II, mouse

The Neuron Isolation Kit, mouse depletes all non-neuronal cells so that "untouched neurons" remain.

Background information

The Neuron Isolation Kit, mouse is an indirect magnetic labeling system for the isolation of untouched neurons from cell suspensions of mouse neural tissue. Non-neuronal cells like astrocytes, oligodendrocytes, microglia, endothelial cells, fibroblasts, except erythrocytes, are indirectly magnetically labeled by using biotin-conjugated antibodies specific for non-neuronal cells in combination with Anti-Biotin MicroBeads. Isolation of highly pure unlabeled neuronal cells is achieved by depletion of the magnetically labeled cells. The cell number and composition of the neuronal cell fraction differs according to the mouse age and the brain region used for cell isolation. The isolation of neurons has been tested with CD-1

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mice aged from embryonic day 18 (E18) to adult. For optimal results, the Neural Tissue Dissociation Kit – Postnatal Neurons or the Adult Brain Dissociation Kit have been used prior to this kit.

Columns

LS or autoMACS

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Columns.

Figure 1

Neuronal cells were isolated from P2 and from 9 weeks old CD-1

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mouse brains. P2 mouse brain was dissociated using the Neural Tissue Dissociation Kit – Postnatal Neurons and adult mouse brain using the Adult Brain Dissociation Kit, mouse and rat. The neurons were isolated using the Neuron Isolation Kit, mouse, LS Columns, and a MidiMACS™ Separator.

Cells were fluorescently stained with an antibody cocktail specific for non-neuronal cells and the astrocyte-specific antibody Anti-ACSA-2-PE, the oligodendrocyte-specific antibody Anti-O4-PE, and the microglia-specific antibody CD11b-FITC. Cells were analyzed by flow cytometry using the MACSQuant™ Analyzer 10. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Neonatal cells