YILIMART - Germinal Center B Cell (PNA) MB Kit, m

B cells within germinal centers express ligands for peanut agglutinin (PNA), allowing for their identification and isolation.

The Germinal Center B Cell (PNA) MicroBead Kit, mouse has been optimized for the rapid and simple isolation of mouse germinal center B cells from single-cell suspensions from lymphoid tissues upon immunization.

Background information

Following primary immunization with T-dependent antigen, B cells in the lymphatic tissues can form germinal centers (GC). Here B cells proliferate, differentiate, and mutate their antibody genes through somatic hypermutation, and switch the class of their antibody.

B cells within GC express ligands for peanut agglutinin (PNA), allowing for their identification and isolation. PNA is a plant lectin protein derived from the fruits of

Arachis hypogae

, which specifically binds to terminal non-reducing galactose residues on the cellular membrane.

Downstream applications

Germinal center B cell isolated with Germinal Center B Cell (PNA) MicroBead Kit, mouse can be used for phenotypical and functional analyses of germinal center B cells, e.g. antibody screening, cytokine secretion, antigen presentation and cell development studies in different experimental murine models of immunization.

Columns

MS or LS Columns.

Figure 1

Isolation of germinal center B cells from mouse SRBC-immunized spleen using the Germinal Center B Cell (PNA) MicroBead Kit, an MS Column, and a MiniMACS™ Separator. Cells are fluorescently stained with CD19-PE-Vio

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770, CD38-APC, and CD95-PE and analyzed by flow cytometer using the MACSQuant

^®

Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Before separation


After separation

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Germinal Center B Cell (PNA) MB Kit, m

For research use only

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Background information

Downstream applications

Columns

Figure 1

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