YILIMART - Anti-F4/80-MicroBeads UltraPure, mouse

In mice F4/80 is a well-established, yet not exclusive, macrophage marker. Anti-F4/80 MicroBeads UltraPure have been optimized for the rapid and simple isolation of mouse macrophages from single-cell suspensions from lymphoid and non-lymphoid tissues. The UltraPure MicroBeads greatly improve recovery and purity of the sorted population by specifically enriching viable cells.

F4/80 is a member of epidermal growth factor (EGF)-transmembrane 7 (TM7) family and consists of a seven-span transmembrane molecule with a large extracellular, multiple EGF module–containing, domain. F4/80 is considered as one of the most specific cell-surface markers for murine macrophages. Constitutive and high expression of F4/80 is found on most resident tissue macrophages, including spleen, microglia in the brain, Kupffer’s cells in the liver, and Langerhans cells in the skin. In addition, the expression of F4/80 can also be regulated depending on the physiological status of the cell.

Downstream applications

Macrophages isolated with Anti-F4/80 MicroBeads UltraPure can be used for phenotypical and functional analyses, e.g. cytokine secretion, phagocytosis, polarization, and tolerance induction in different experimental murine models.

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MS, LS, or autoMACS

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Figure 1

F4/80

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cells were isolated from spleen single-cell suspension using Anti-F4/80 MicroBeads UltraPure, two MS Columns, and an OctoMACS™ Separator. The cells were fluorescently stained with CD45-VioBlue

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, CD11b-APC, and Anti-F4/80-FITC and analyzed by flow cytometry using the MACSQuant

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Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and 7-AAD fluorescence.

Before separation	F4/80 ⁺ cells

Selected references
1. Wynn, T.A. et al. (2013) Macrophage biology in development, homeostasis anddisease. Nature 496: 445-455
2. Austyn, J. M. et al. (1981) F4/80, a monoclonal antibody directed specifically against the mouse macrophage. Eur. J. Immunol. 11: 805-815
Brochures and posters

Isolation and analysis of murine myeloid cells from lymphoid tissues and tumor

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Anti-F4/80-MicroBeads UltraPure, mouse

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Downstream applications

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Figure 1

Selected references

Brochures and posters

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