Background information

Columns

• Figure 1

  EpCAM + human breast carcinoma cells were isolated from a heterogeneous sample of human cells using the Tumor Cell Isolation Kit, human, an LS Column, and a QuadroMACS™ Separator after the tissue was dissociated using the Tumor Dissociation Kit, human and the gentleMACS™ Octo Dissociator. The cells were fluorescently stained with CD326 (EpCAM)‑VioBlue ® and CD31‑PE and analyzed by flow cytometry using the MACSQuant ® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

  Before separation	Isolated human tumor cells

• Figure 2

  EpCAM positive human breast carcinoma cells were isolated from a heterogeneous sample of human cells using the Tumor Cell Isolation Kit, human, an LS Column, and a QuadroMACS™ Separator. Subsequently, the unseparated fraction and the negative fraction were cultured for seven days in expansion medium (90% RPMI-1640, 2mM L-Glutamine, 10% FBS, 100 U/mL penicillin, and 100 U/mL streptomycin). After fixation, cells were stained using antibodies specific for vimentin (red), labeling fibroblasts, and EpCAM (green), labeling tumor cells. Cell nuclei were stained with DAPI (blue).

  Before separation	Isolated human tumor cells

• Scientific posters

  Isolation of primary human tumor cells improves culture of target cells and reduces bias in molecular analysis
  Optimized workflow improves the characterization of tumor-infiltrating T cells. SITC (2017)
  Tumor Cell Isolation, human - AACR 2016
  Tumor cells from human, mouse, and xenografted tumors - EACR 2016