YILIMART - Anti-PE MicroBeads UltraPure

Anti-Phycoerythrin (PE) MicroBeads are used for the indirect magnetic labeling and separation of cells or other materials labeled with a PE-conjugated primary antibody or ligand. The use of PE-conjugated primary antibodies and Anti-PE MicroBeads leads to the highest purity and recovery, even when the marker used for sorting is weakly expressed. Anti-PE MicroBeads UltraPure have been especially designed for cell separations from debris-rich biological starting materials.

Background information

Anti-PE MicroBeads UltraPure have been especially developed for highly efficient separation of cells from debris-rich samples or other biological materials according to surface markers labeled with PE-conjugated primary antibodies, peptides or ligands. After separation the PE-labeled cells can be directly detected by flow cytometry or fluorescence microscopy without any further staining. Together with its ultrapure cell separation result even from debris-rich starting material Anti-PE MicroBeads UltraPure are the latest development of Anti-PE MicroBeads.

Applications

Anti-PE MicroBeads are well-established as a component of the Cytokine Secretion Assays for the enrichment of antigen-specific T cells. They have also been used to isolate antigen-specific T cells after labeling with PE-conjugated MHC Class I tetramers

^1,2

or MHC Class II tetramers

³

. Moreover, they have been used to separate a CD62L

⁺

subset of CD4

⁺

CD45RO

⁺

memory T cells from human adenoids, which had been pre-sorted by MACS® Technology

⁴

. The latest development of Anti-PE MicroBeads UltraPure enables high-yield positive cell separation using any PE-labeled primary antibody, even from low quality and rare cell samples.

Columns

For positive selection: MS, LS, XS, or autoMACS

^®

Columns. For depletion: LD, D, or autoMACS Columns.

Figure 1

Isolation of cytomegalovirus (CMV)-specific T cells from PBMCs using PE-conjugated CMVpp65/A2 tetramers (courtesy of Prof. Moss, Birmingham, U.K.) and Anti-PE MicroBeads. Cells were positively selected by separation over two MS Columns.

PBMCs before separation	Enriched CMV-specific T cells

Figure 2

Human CLEC9a

⁺

cells have been isolated from a debris-rich sample of peripheral blood mononuclear cells (PBMCs) using Anti-CLEC9a-PE, Anti-PE MicroBeads UltraPure, two MS Columns, and a MiniMACS™ Separator. Cells were stained with Anti-CLEC9a-PE (# 130-097-368), CD141 (BDCA-3)-APC (# 130-090-907), and Propidium Iodide Solution (# 130-093-233). Cells were analyzed after gating on viable lymphoid cells.

Before separation	After separation

Selected references
1. Shankar et al. (2001) Blood 96: 3094-3101
2. Barnes et al. (2004)
  
  Ultra-sensitive class I tetramer analysis reveals previously undetectable populations of antiviral CD8
  ⁺
  T cells.
  
  Eur. J. Immunol. 34: 1570-1577
3. Lucas, M. et al. (2004)
  Ex vivo
  phenotype and frequency of influenza virus-specific CD4 memory T cells.
  
  J. Virol. 78: 7284-7287
4. Mattila, P. S. et al. (2000)
  
  Adenoids provide a microenvironment for the generation of CD4
  ⁺
  , CD45RO
  ⁺
  , L-selectin
  ^–
  , CXCR4
  ⁺
  , CCR5
  ⁺
  T lymphocytes, a lymphocyte phenotype found in the middle ear effusion.
  
  Int. Immunol. 12: 1235-1243

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Anti-PE MicroBeads UltraPure

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Background information

Applications

Columns

Figure 1

Figure 2

Selected references

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