Detailed separation procedure

Downstream applications

Columns

• Figure 1

  A single-cell suspension from mouse spleen was prepared using the program m_spleen_01.01 on the gentleMACS Dissociator. CD4 + T cells were isolated from this single-cell suspension using the CD4 + T Cell Isolation Kit, an LS Column, and a MidiMACS™ Separator. Cells were fluorescently stained with the MC CD4 T Cell Cocktail (# 130-092-916) and analyzed by flow cytometry using the MACSQuant® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

  Unseparated fraction	CD4 + T cells after depletion of non-CD4 + T cells

• Brochures and posters

  MACS Cell Pre-Enrichment for flow sorting

• Further downloads

  Expansion of mouse CD4

  ⁺

  T cells