YILIMART - Pan B Cell Isolation Kit II, mouse

Pan B Cell Isolation Kit II, mouse

For research use only

货号：: 130-104-443
描述：: Pan B Cell Isolation Kit II, mouse
包装：: for 1×10⁹ total cells

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The Pan B Cell Isolation Kit II was developed for the fast and efficient isolation of untouched murine B-1 and B-2 B cell subsets from single-cell suspensions of lymphoid tissues.

Detailed separation procedure

For isolation of target cells, non-B cells are labeled with a cocktail of biotinylated CD3ε, CD4, CD8a, CD49b, Gr-1, and Ter119 antibodies. These cells are subsequently magnetically labeled with Anti-Biotin MicroBeads. Highly pure B cells are obtained by depletion of the magnetically labeled cells. The kit does not contain CD43 or CD11b in the depletion cocktail which might be expressed on some malignant target cells. It is therefore perfectly suited to be used for isolation of B cells from different mouse models for human diseases, e.g. B cells from B-CLL mouse models.

Columns

MS, LS, XS, or autoMACS

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Columns.

Figure 1

Pan B cells were isolated from spleen of a BALB/c mouse by using the Pan B Cell Isolation Kit II, an LS Column, and a MidiMACS™ Separator. The cells were fluorescently stained with CD19-APC (# 130-102-494) and CD45-FITC (# 130-102-491) and analyzed by flow cytometry using the MACSQuant® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Unseparated fraction	After separation

Brochures and posters

MACS Cell Pre-Enrichment for flow sorting

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