Background information

Downstream applications

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• Figure 1

  A single-cell suspension from mouse spleen was prepared using the program m_spleen_01.01 on the gentleMACS™ Dissociator. CD8a + T cells were isolated from this single-cell suspension using the CD8a+ T Cell Isolation Kit, an LS Column, and a MidiMACS™ Separator. Cells were fluorescently stained with the MC CD8a T Cell Cocktail (# 130-092-915) and analyzed by flow cytometry using the MACSQuant® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

  Unseparated fraction:	Isolated CD8 + T cells:

• Selected references

  1. Hamilton-Williams, E. E. et al. (2009) Expression of diabetes-associated genes by dendritic cells and CD4 T cells drives the loss of tolerance in nonobese diabetic mice. J. Immunol. 183: 1533-1541
  2. Grebe, K. M. et al. (2009)
     Sympathetic nervous system control of anti-influenza CD8

     ⁺

     T cell responses.
     Proc. Natl. Acad. Sci. U.S.A. 106: 5300-5305
  3. Simma et al. (2009) Identification of an indispensable role for tyrosine kinase 2 in CTL-mediated tumor surveillance. Cancer Res. 69: 203-211