YILIMART - Anti-ROR1 MicroBead Kit, human

The Anti-ROR1 MicroBead Kit was developed for the positive selection or depletion of cells expressing human ROR1 antigen.

Background information

ROR1 has characteristics of an oncofetal gene and was recently identified to be overexpressed at the gene and protein levels in chronic lymphocytic leukemia (CLL) and mantle cell lymphoma (MCL). ROR1 expression has also been detected in ovarian cancer, renal cancer, melanoma, and lung adenocarcinoma, suggesting a general role of ROR1 in cancer genesis and/or maintenance.

Moreover, to a much lower level ROR1 expression has been found in undifferentiated embryonic stem cells, in adipose tissue, in early stage of B cell development, but not in major adult tissues. In blood samples with low frequencies of CLL B cells, e.g., in minimal residual disease, the enrichment of these cells via the Anti-ROR1 MicroBead Kit allows analysis and downstream processing of these cells.

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MS, LS, or autoMACS

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Figure 1

ROR1

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cells were isolated from PBMCs (that were previously prepared from B-CLL blood samples) using the Anti-ROR1 MicroBead Kit, an LS Column, and a MidiMACS

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Separator. Cells were fluorescently stained with CD19-APC (# 130-091-248) and analyzed by flow cytometry using the MACSQuant

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Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Unseparated fraction	ROR1 ^- cells	ROR1 ⁺ cells

Customer reports and application notes

Sensitive analysis and isolation of ROR1
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B cells for research on chronic lymphocytic leukemia

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Anti-ROR1 MicroBead Kit, human

For research use only

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Background information

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Figure 1

Customer reports and application notes

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