YILIMART - CD171 (L1CAM) MicroBead Kit, mouse

CD171 (L1CAM) MicroBead Kit was developed for the direct isolation or depletion of CD171 (L1CAM)

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neurons from dissociated mouse brain tissue derived from mice younger than eight days (

Background information

CD171 (L1CAM) MicroBeads (L1CAM: L1 cell adhesion molecule) have been developed for the separation of mouse cells based on the expression of the CD171 (L1CAM) antigen. Expression of the cell adhesion molecule CD171 is found on tetanus-toxin positive neurons

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, endothelial cells, certain epithelial cells, reticular fibroblasts, and several malignant tumors including colon and breast carcinomas, colon melanoma, tumor cells of neuronal and mesothelial origin, where a role of CD171 in augmenting tumor growth has been demonstrated. CD171 plays a vital role in cell adhesion and signal transduction. It is involved in the development of the nervous system and regulates processes such as neuron–neuron adhesion, myelination, axonal guidance, and neuronal migration.

CD171 (L1CAM) MicroBeads were especially optimized for the separation of neurons based on the expression of CD171 (L1CAM). The isolation was optimized on dissociated postnatal CD-1

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mouse brain tissue derived from animals younger than postnatal day eight (P8). Up to 99.5% purity was obtained with P7 cerebellum tissue because here the expression of CD171 is found almost exclusively on neurons. Purity decreases with age: P9 up to 80%; P12 up to 64%.

For other brain regions, the depletion of glial cells might become necessary if high purities are required.

The CD171 (L1CAM) epitope shows papain sensitivity. Therefore, the Neural Tissue Dissociation Kit (T) is recommended prior to cell isolation.

Figure 1

CD171 (L1CAM)

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cells were isolated from P1 CD-1 mouse cerebellum tissue using the Neural Tissue Dissociation Kit (T), the gentleMACS Dissociator, the CD171 (L1CAM) MicroBead Kit, a MidiMACS™ Separator, and an LS Column. Cells were fluorecently stained with Labeling Check Reagent-APC (# 130-095-237) and analyzed by flow cytometry using the MACSQuant

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Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Before separation	CD171 (L1CAM) ⁺ cells	CD171 (L1CAM) ^- cells

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CD171 (L1CAM) MicroBead Kit, mouse

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Background information

Figure 1

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