Background information

Applications

Columns

• Figure 1

  Pluripotent (TRA-1-60 + ) iPSCs were isolated from cultures containing spontaneously differentiated iPSCs grown on mouse embryonic feeder cells using Anti-TRA-1-60 MicroBeads, an MS Column, and a MiniMACS™ Separator. Cells were fluorescently stained with Anti-TRA-1-60-PE (# 130-100-347) and Anti-Feeder-APC after separation and analyzed by flow cytometry using the MACSQuant ® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

  Unseparated fraction	Enriched TRA-1-60 + pluripotent stem cells

• Selected references

  1. Dick, E. et al. (2011) Faster generation of hiPSCs by coupling high-titer lentivirus and column-based positive selection. Nat. Protoc. 6(6): 701-714
  2. Andrews, P. W. et al. (1984) Three monoclonal antibodies defining distinct differentiation antigens associated with different high molecular weight polypeptides on the surface of human embryonal carcinoma cells. Hybridoma 3: 347-361
  3. Chan, E. M. et al. (2009) Live cell imaging distinguishes bona fide human iPS cells from partially reprogrammed cells. Nat. Biotechnol. 27: 1033-1037
  4. Grabundzija, I. et al. (2013) Sleeping Beauty transposon-based system for cellular reprogramming and targeted gene insertion in induced pluripotent stem cells. Nucleic Acids Res. 41(3): 1829-1847
  5. Tanabe, K. et al. (2013) Maturation, not initiation, is the major roadblock during reprogramming toward pluripotency from human fibroblasts. Proc. Natl. Acad. Sci. U.S.A. 110: 12172-12179

• Scientific posters

  Sequential enrichment of TRA-1-60

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  PSC and CD271 (p75)

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  NCSC enhances peripheral neuron differentiation

• Customer reports and application notes

  Isolation of human induced pluripotent stem cell lines. Dick et al. (2012) MACS&more 14(2).