YILIMART - Anti-Ly6b.2 (7/4) MicroBeads, mouse

The Anti-Ly6B.2 (7/4) MicroBeads, mouse can be used for isolation of neutrophils, inflammatory monocytes or myeloid progenitors from bone marrow or spleen.

Background information

Positive selection or depletion of cells expressing mouse Ly-6B.2 antigen from bone marrow and spleen.
Isolation of recruited inflammatory myeloid cells in experimental mouse models. The antigen is only expressed on mouse strains of the Ly6.2 haplotype.

Detailed separation procedure

First, the Ly-6B.2 (7/4)

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cells are magnetically labeled with Anti- Ly-6B.2 (7/4) MicroBeads. Then, the cell suspension is loaded onto a MACS

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Column, which is placed in the magnetic field of a MACS Separator. The magnetically labeled Ly-6B.2 (7/4)

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cells are retained within the column. The unlabeled cells run through; this cell fraction is thus depleted of Ly-6B.2 (7/4)

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cells. After removing the column from the magnetic field, the retained Ly-6B.2 (7/4)

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cells can be eluted as the positively selected cell fraction. To increase the purity of spleen cells, the positively selected cell fraction containing the Ly-6B.2 (7/4)

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cells must be separated over a second column.

Columns

MS, LS, or autoMACS

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Columns.

Figure 1

Ly-6B.2 (7/4)

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cells were isolated from spleen single-cell suspension using Anti-Ly-6B.2 (7/4) MicroBeads, 2 MS Columns and an OctoMACS™ Separator. The cells were fluorescently stained with CD11b-APC (# 130-098-088) and Anti-Ly-6B.2 (7/4)-PE (# 130- 098-801) and analyzed by flow cytometry using the MACSQuant

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Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Unseparated fraction	Ly-6B.2 (7/4) ⁺ cells

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Anti-Ly6b.2 (7/4) MicroBeads, mouse

For research use only

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Background information

Detailed separation procedure

Columns

Figure 1

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