Detailed separation procedure

Downstream applications

Columns

• Figure 1

  Pre-enrichment of untouched Pan-DCs from human PBMCs using the Pan-DC Enrichment Kit, human, a MidiMACS Separator and an LS Column. The cells were fluorescently stained with CD303 (BDCA-2)-FITC, CD141 (BDCA-3)-APC, CD1c (BDCA-1)-PE, and CD20-PerCP and analyzed by flow cytometry using the MACSQuant® Analyzer. Cell debris, dead cells, and B cells were excluded from the analysis based on scatter signals, propidium iodide fluorescence, and CD20 expression.

  Unseparated fraction

  Enriched Pan-DCs

  Non-DCs

• Selected references

  1. O’Doherty, U. et al. (1994) Human blood contains two subsets of dendritic cells, one immunologically mature and the other immature. Immunology 82: 487-493
  2. Dzionek, A. et al. (2000) BDCA-2, BDCA-3, BDCA-4: Three markers for distinct subsets of dendritic cells in human peripheral blood. J. Immunol. 165: 6037-6046
  3. Penna, G. et al. (2001) Cutting Edge: Selective usage of chemokine receptors by plasmacytoid dendritic cells.. J. Immunol. 167: 1862-1866
  4. Penna, G. et al. (2002) Cutting edge: differential chemokine production by myeloid and plasmacytoid dendritic cells. J. Immunol. 169: 6673-6676
  5. Krug, A. et al. (2001) Toll-like receptor reveals CpG DNA as a unique microbial vstimulus for plasmacytoid dendritic cells which synergizes with CD40 ligand to induce high amounts of IL-12. Eur. J. Immunol. 31: 3026-3037