YILIMART - Monocyte Isolation Kit (BM), mouse

Monocyte Isolation Kit (BM), mouse

For research use only

货号：: 130-100-629
描述：: Monocyte Isolation Kit (BM), mouse
包装：: for 1×10⁹ total cells

- +

添加到购物车

您浏览过的商品

The Monocyte Isolation Kit (BM) has been developed for the easy isolation of monocytes from mouse bone marrow by depletion of unwanted cells.

Detailed separation procedure

Using the Monocyte Isolation Kit (BM), mouse, monocytes are isolated by depletion of non-target cells. Non-target cells are indirectly magnetically labeled with a cocktail of biotin-conjugated monoclonal antibodies, as primary labeling reagent, and anti-biotin monoclonal antibodies conjugated to MicroBeads as secondary labeling reagent. The unwanted cells are depleted by retaining them within a MACS Column in the magnetic field of a MACS Separator, while the unlabeled monocytes pass through the column.

Downstream applications

Isolation of mouse monocytes from mouse bone marrow for in vitro analysis, differentiation studies, co-culture or adoptive transfer experiments.

Columns

LS or autoMACS

^®

Columns.

Figure 1

Monocytes were isolated from C57BL/6 bone marrow cells by using the Monocyte Isolation Kit (BM), mouse, an LS Column and a QuadroMACS Separator. The cells were fluorescently stained with CD11b-VioBlue, Lin cocktail (CD3ε-PE, CD45R (B220)-PE, Anti-NK1.1-PE, Anti-Ly-6G-PE, CD49b-PE), Anti-Ly-6C-FITC and Anti-Siglec-F-APC and analyzed by flow cytometry using the MACSQuant Analyser. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Unseparated fraction


Isolated monocytes

查看数据文件查看下载