YILIMART - CD34 MicroBead Kit UltraPure, human

The CD34 MicroBead Kit UltraPure is the latest development for positive selection of CD34+ hematopoietic stem and progenitor cells. It is suited for all routine CD34 cell isolations and is the ideal choice for debris-rich, low frequency samples.

Background information

CD34 is a well-established marker of human hematopoietic stem and progenitor cells and additionally expressed on hemangioblasts, endothelial progenitor cells, and mature endothelial cells.

The CD34 MicroBead Kit UltraPure contains MicroBeads directly conjugated to CD34 antibodies for magnetic labeling of CD34-expressing cells from peripheral blood, cord blood, bone marrow, or apheresis harvest. Hematopoietic progenitors present at a frequency of 0.05-0.2% among peripheral blood mononuclear cells (PBMCs), 0.1-0.5% among cord blood mononuclear cells (MNCs) or 0.5-3% among bone marrow MNCs can be rapidly and efficiently enriched.

The CD34 MicroBead Kit UltraPure is suited for all routine CD34+ cell isolations. In addition, its unique formulation provides particular advantages with debris-rich sample material.

Control staining of cells isolated with the CD34 MicroBead Kit requires a monoclonal CD34 antibody other than QBEND/10, e.g., clone AC136.

Downstream applications

CD34⁺ cell expansion in culture¹
Lentiviral transduction¹
Molecular analysis of leukemic stem cells, e.g. AML blasts or CD34+ myelofibrosis samples²
In vitro differentiation of CD34+ cells³^,4
Isolation of endothelial progenitor cells⁵
Generation of humanized mice⁶

Columns

MS, LS, XS, or autoMACS

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Figure 1

Isolation of CD34

⁺

cells from a debris-rich PBMC sample. Cells were stained with CD34-PE, CD45-FITC and Propidium Iodide Solution. Cells before and after separation were analyzed by gating on PI-, lymphoid cells.

Unseparated fraction	After separation

Figure 2

A comparison of the ungated forward scatter versus side scatter profile of the same sample after separation with the CD34 MicroBead Kit (# 130-046-702) or the CD34 MicroBead Kit UltraPure (# 130-100-453) demonstrates reduced debris carry-over with the CD34 MicroBead Kit UltraPure.

CD34 MicroBead Kit	CD34 MicroBead Kit UltraPure

Selected references
1. Venton, G. et al. (2016) Aldehyde dehydrogenases inhibition eradicates leukemia stem cells while sparing normal progenitors. Blood Cancer J. 6(9): 469
2. van Til, N.P. and Wagemaker, G. (2014) Lentiviral gene transduction of mouse and human hematopoietic stem cells. Methods Mol. Biol. 1185: 311-319
3. Harada-Shirado, K. et al. (2015) Dysregulation of the MIRLET7/HMGA2 axis with methylation of the CDKN2A promoter in myeloproliferative neoplasms. Br. J. Haematol. 168(3): 338-349
4. Giani, F.C. et al. (2016) Cell Stem Cell 18: 73-78
5. Moretta, F. et al. (2016) The generation of human innate lymphoid cells is influenced by the source of hematopoietic stem cells and by the use of G-CSF. Eur. J. Immunol. (DOI: 10.1002/eji.201546079)
6. Campesi, I. et al. (2015) Estrogenic compounds have divergent effects on human endothelial progenitor cell migration according to sex of the donor. J. Vasc. Res. 52(4): 273-278
7. Futrega K et al. (2016) Direct bone marrow HSC transplantation enhances local engraftment at the expense of systemic engraftment in NSG mice. Sci. Rep. 6: 23886
Brochures and posters

CD34 MicroBead Kit UltraPure

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CD34 MicroBead Kit UltraPure, human

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Background information

Downstream applications

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Figure 1

Figure 2

Selected references

Brochures and posters

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