Background information

Applications

Columns

• Figure 1

  Plasma cells were isolated from mouse spleen (A, B) or mouse bone marrow (C, D) using CD138 MicroBeads, two MS Columns per separation, and a MiniMACS™ Separator. Cells were fluorescently stained with CD19-APC (# 130-092-039) and CD138-PE and analyzed by flow cytometry using the MACSQuant ® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence. Note that CD138 ++ cells represent the plasma cell fraction in both tissues. CD138 + cells in bone marrow are B cell precursors.

  Spleen

  A:	B:
  Before enrichment	After enrichment
  Bone marrow

  C:	D:
  Before enrichment	After enrichment