Background information

Columns

• Figure 1

  SSEA-4 expressing cells were isolated from a heterogeneous suspension of U937 cells (SSEA-4 – CD326 – ) and NTERA cells (SSEA-4 + CD326 + ) using Anti-SSEA-4 MicroBeads, an LS Column, and a MidiMACS Separator. Cells were fluorescently stained with Labeling Check Reagent-FITC and CD326 (EpCAM)-APC. Cell debris and dead cells were excluded based on scatter signals and propidium iodide fluorescence.

  Unseparated fraction	After separation

• Selected references

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  2. Valamehr, B. et al. (2012) A novel platform to enable the high-throughput derivation and characterization of feeder-free human iPSCs. Sci. Rep. 2: 213
  3. Kannagi, R. et al. (1983) Stage-specific embryonic antigens (SSEA-3 and -4) are epitopes of a unique globo-series ganglioside EMBO J. 2(12): 2355-2361
  4. Shamblott, M. J. et al. (1999) Proc. Natl. Acad. Sci. U.S.A. 95: 13726-13731
  5. Bhartiya, D. et al. (2012) Very small embryonic-like stem cells with maximum regenerative potential get discarded during cord blood banking and bone marrow processing for autologous stem cell therapy. Stem Cells Dev. 21: 1-6
  6. D'Ippolito, G. et al. (2006) Sustained stromal stem cell self-renewal and osteoblastic differentiation during aging. Rejuvenation Res. 9: 10-19