Background information

• Figure 1

  ACSA-2 + cells were isolated from P4 CD-1 mouse brain tissue using the Neural Tissue Dissociation Kit (P), the gentleMACS™ Dissociator, FcR Blocking Reagent, mouse, Anti-ACSA-2 MicroBeads, a MiniMACS™ Separator, and an MS Column. Cells were fluorecently stained with Anti-ACSA-2-PE and analyzed by flow cytometry using the MACSQuant ® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

  Before separation	ACSA-2 + cells
  ACSA-2 – cells

• Selected references

  1. Feldmann, M. et al. (2014) Isolating astrocytes and neurons sequentially from postnatal murine brains with a magnetic cell separation technique. J. Biol. Methods 1(2): e11
  2. Sharma, K. et al. (2015) Cell type- and brain region-resolved mouse brain proteome. Nat. Neurosci. 18(12): 1819-1831
  3. Holt, L. M. and Olsen, M. L. (2016) Novel applications of magnetic cell sorting to analyze cell-type specific gene and protein expression in the central nervous system. PLoS One 11(2): e0150290
  4. G. Kantzer, C. et al. (2017) Anti-ACSA-2 defines a novel monoclonal antibody for prospective isolation of living neonatal and adult astrocytes. Glia 65(6): 990-1004
  5. Batiuk, M. Y. et al. (2017) An immunoaffinity-based method for isolating ultrapure adult astrocytes based on ATP1B2 targeting by the ACSA-2 antibody. J. Biol. Chem. 292(21): 8874-8891

• Scientific posters

  Efficient isolation of viable primary neural cells from adult murine brain tissue based on a novel automated tissue dissociation protocol. Zhang, H. et al. SfN (2016)
  Highly efficient dissociation of distinct adult rodent brain regions ensures high cell viability and recovery for successful downstream processing. Reiß, S. et al. FENS (2018)
  Characterization of the cytokine secretion profile of highly purified, functional astrocytes. XII European Meeting on Glial Cells in Health and Disease (2015)
  A novel dissociation procedure allows efficient immunomagnetic isolation of astrocytes, oligodendrocytes, and neurons from adult rodent brain tissue. XII European Meeting on Glial Cells in Health and Disease (2015)

• Special protocols

  Isolation of astrocytes from adult mouse brain

• Further downloads

  Materials required for the isolation, analysis, and culture of astrocytes from adult mouse brain