YILIMART - Naive Pan T Cell Isolation Kit, human

The Naive Pan T Cell Isolation Kit was developed for the fast and efficient isolation of untouched naive T cells from human PBMCs.

Detailed separation procedure

For depletion of memory T cells and non-T cells, PBMCs are incubated with a cocktail of biotinylated CD45RO, CD14, CD15, CD16, CD19, CD25, CD34, CD36, CD57, CD123, anti-HLA-DR, CD235a (Glycophorin A), and CD244 antibodies. Optionally, biotinylated Anti-TCRγ/δ antibodies can be added for depletion of TCRγ/δ

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T cells. The cells are subsequently magnetically labeled with Anti-Biotin MicroBeads. CD61 MicroBeads are added for concurrent depletion of platelets.

Downstream applications

Naive pan T cells isolated with the Naive Pan T Cell Isolation Kit can be used when direct labeling of cell surface molecules could interfere with downstream applications, e.g., in studies on naive T cell activation and in functional studies of DCs. Untouched naive pan T cells can also be used for further isolation of specific T cell subsets.

Columns

LS or autoMACS

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Columns.

Figure 1

Separation of untouched naive T cells from PBMCs by depletion of non-T cells using the Naive Pan T Cell Isolation Kit, an LS Column, and a MidiMACS™ Separator. Cells were labeled with CD3-VioBlue, CD197-PE and CD45RA-FITC and analyzed by flow cytometry using the MACSQuant® Analyzer.

PBMCs before separation


Untouched naive T cells

Selected references
1. Che et al. (2010)
  
  HIV-1 impairs
  in vitro
  priming of naive T cells and gives rise to contact-dependent suppressor T cells.
  
  Eur. J. Immunol. 40: 2248-2252
2. Gattinoni, L. et al. (2011) A human memory T cell subset with stem cell-like properties. Nat. Med. 17: 1290-1297
3. Jedema, I. et al. (2011) Successful generation of primary virus-specific and anti-tumor T-cell responses from the naive donor T-cell repertoire is determined by the balance between antigen-specific precursor T cells and regulatory T cells. Haematologica 96(8): 1204-1212

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Naive Pan T Cell Isolation Kit, human

For research use only

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Detailed separation procedure

Downstream applications

Columns

Figure 1

Selected references

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