YILIMART - CD19 MicroBeads, human

CD19 MicroBeads were developed for positive selection or depletion of CD19

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B cells from different cell sources such as human PBMCs, bone marrow, lymphoid tissues, or cell cultures.

Background information

As a B cell lineage marker, the CD19 antigen is expressed from the early pro-B cell stage to the B cell lymphoblast stage, but the expression is down-regulated upon B cell maturation to plasma cells. The CD19 antigen is further expressed on most malignant B cells and on a subset of follicular dendritic cells.

Downstream applications

Isolated CD19

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B cells were used for studies on immunoglobulin production, such as IgE synthesis

^{1, 2}

and secretion

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, chemotaxis assays

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, or mixed lymphocyte reactions. Positively selected CD19

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B cells were also used for molecular studies such as gene expression profiling.

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For the analysis of minimal residual diseases in B cell malignancies, enrichment of B cells increases the sensitivity of detection.

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Columns

For positive selection: MS, LS, XS, or autoMACS

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Columns. For depletion: LD, D, or autoMACS Columns.

Figure 1

CD19

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cells were isolated from human PBMCs using CD19 MicroBeads, an MS Column, and a MiniMACS™ Separator. Cells were fluorescently stained with CD19-PE and analyzed by flow cytometry. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Unseparated fraction	After separation

Figure 2
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Figure 2

Separation of CD19
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cells from PBMCs using CD19 MicroBeads, an MS Column, and a MiniMACS™ Separator.

Separation of CD19
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cells from PBMCs using CD19 MicroBeads, an MS Column, and a MiniMACS™ Separator.

Selected references
1. Aebischer et al. (1994)
  
  Neuropeptides are potent modulators of human
  in vitro
  immunoglobulin E synthesis.
  
  Eur. J. Immunol. 24: 1908-1913
2. Stämpfli et al. (1994) Inhibition of human IgE synthesis by anti-IgE antibodies requires divalent recognition. Eur. J. Immunol. 24: 2161-2167
3. Zürcher, A. W. et al. (1995) IgE-producing hybridomas established after B-cell culture in the CD40 system. Immunol. Lett. 46: 49-57
4. Schratzberger, P. et al. (1997) Differential chemotactic activities of sensory neuropeptides for human peripheral blood mononuclear cells. J. Immunol. 158: 3895-3901
5. Klein, U. et al. (2001) Gene expression profiling of B cell chronic lymphocytic leukemia reveals a homogeneous phenotype related to memory B cells. J. Exp. Med. 194: 1625-1638
6. Pugh, R. E. et al. (1998) CD19 selection improves the sensitivity of B cell lymphoma detection. J. Hematother. 7: 159-168
Brochures and posters

MACS Cell Pre-Enrichment for flow sorting

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CD19 MicroBeads, human - lyophilized

For research use only

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Background information

Downstream applications

Columns

Figure 1

Figure 2

Figure 2

Selected references

Brochures and posters

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