YILIMART - Anti-FITC MicroBeads -lyophilized

Anti-FITC MicroBeads are widely used for indirect magnetic labeling and separation of cells or other materials which are labeled with a primary antibody or ligand conjugated to fluorescein isothiocyanate (FITC).

Background information

The FITC molecule is recognized by the monoclonal anti-FITC antibody coupled to MicroBeads, allowing the positive selection or depletion of the fluorescently labeled material with MACS

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Technology. Labeling samples with FITC-conjugated primary antibodies and Anti-FITC MicroBeads enables subsequent flow cytometric analysis of separated cells without any further staining of the material.

Applications

Examples among the many separations performed with Anti-FITC MicroBeads are the isolation of IgD

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B cells from human PBMCs

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, the separation of plant protoplasts

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as well as the isolation of murine Langerhans cells from epidermal tissue

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. Anti-FITC MicroBeads have also been used for the depletion of CD3

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cells from rat spleen and subsequent isolation of CD3

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NK cells

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or the separation of murine naive CD8

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T cells expressing low levels of CD44 by depletion of CD44

^int/high

cells

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.

Columns

For positive selection: MS, LS, XS, or autoMACS

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Columns. For depletion: LD, D, or autoMACS Columns.

Figure 1
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Figure 1

Erythrocytes from sickle cell anemia patients isolated using Annexin V-FITC and Anti-FITC MicroBeads.
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(Courtesy of Dr. Kuypers, Oakland, USA.)

Erythrocytes from sickle cell anemia patients isolated using Annexin V-FITC and Anti-FITC MicroBeads.
⁴
(Courtesy of Dr. Kuypers, Oakland, USA.)

Selected references
1. Zan, H. et al. (1998) CD40 engagement triggers switching to IgA1 and IgA2 in human B cells through induction of endogenous TGF-beta: evidence for TGF-beta but not IL-10-dependent direct S mu-->S alpha and sequential S mu-->S gamma, S gamma-->S alpha DNA recombination. J. Immunol. 161: 5217-5225
2. Barth, S. et al. (1994) Selection and enrichment of differentially labeled plant protoplasts. J. Biochem. Biophys. Methods 29: 83-86
3. Saeki, H. et al. (2000)
  
  A migratory population of skin-derived dendritic cells expresses CXCR5, responds to B lymphocyte chemoattractant
  in vitro
  , and co-localizes to B cell zones in lymph nodes
  in vivo
  .
  
  Eur. J. Immunol. 30(10): 2808-2814
4. Kuypers, F. A. et al. (1996) Detection of altered membrane phospholipid asymmetry in subpopulations of human red blood cells using fluorescently labeled annexin V. Blood 87: 1179-1187
5. Mikus, L.D. et al. (2001) Reduced interferon-gamma secretion by natural killer cells from rats susceptible to postviral chronic airway dysfunction. Am. J. Respir. Cell Mol. Biol. 24: 74-82
6. Schüler, T. et al. (2004) Cutting edge: IL-7-dependent homeostatic proliferation of CD8+ T cells in neonatal mice allows the generation of long-lived natural memory T cells. J. Immunol. 172: 15-19

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Anti-FITC MicroBeads -lyophilized

For research use only

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Background information

Applications

Columns

Figure 1

Figure 1

Selected references

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