YILIMART - Pan Monocyte Isolation Kit, human

The Pan Monocyte Isolation Kit is an indirect magnetic labeling system for the isolation of untouched monocytes from human PBMCs. This Kit allows the simultaneous enrichment of classical (CD14

⁺⁺

CD16

^–

), non classical (CD14

⁺

CD16

⁺⁺

) and intermediate (CD14

⁺⁺

CD16

⁺

) monocytes.

¹

Highly pure unlabeled monocytes are obtained by depletion of the magnetically labeled cells.

Detailed separation procedure

Non-monocytes, such as T cells, NK cells, B cells, dendritic cells, and basophils are indirectly magnetically labeled using a cocktail of biotin-conjugated antibodies and Anti-Biotin MicroBeads.

Downstream applications

Functional studies on monocytes, in which any effect due to antibody binding to target cells should be avoided.
Studies on monocyte activation, differentiation, cytokine secretion etc.
Studies on signal transduction in monocytes.
Studies on antigen uptake (e.g. phagocytosis) and antigen presentation by monocytes.
In vitro differentiation of blood monocytes into dendritic cells.

Columns

MS, LS, XS, or autoMACS

^®

Columns.

Figure 1

Isolation of untouched monocytes from PBMCs by using the Pan Monocyte Isolation Kit and an LS Column. Cells were fluorescently stained with CD14-FITC, CD16-PE, and Anti-Slan (M-DC8)-APC (# 130-093-031) and analyzed using the MACSQuant® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and PI fluorescence.

PBMCs before separation


Isolated monocytes

Selected references
1. Ziegler-Heitbrock et al. (2010) Nomenclature of monocytes and dendritic cells in blood. Blood 116: e74-80

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Pan Monocyte Isolation Kit, human

For research use only

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Detailed separation procedure

Downstream applications

Columns

Figure 1

Selected references

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