YILIMART - Pan T Cell Isolation Kit, human

The Pan T Cell Isolation Kit has been developed for the faster isolation of untouched T cells from human peripheral blood mononuclear cells (PBMCs). This updated kit gives you even higher purity, greater recovery, and faster cell isolation. You can isolate untouched T cells in as little as 18 minutes!

Detailed separation procedure

Non-target cells, i.e., monocytes, neutrophils, eosinophils, B cells, stem cells, dendritic cells, NK cells, granulocytes, or erythroid cells are labeled by using a cocktail of biotin-conjugated antibodies. The cocktail contains antibodies against CD14, CD15, CD16, CD19, CD34, CD36, CD56, CD123, and CD235a (Glycophorin A). Subsequently, non-target cells are magnetically labelled with the Pan T Cell MicroBead Cocktail. Isolation of highly pure T cells is achieved by depletion of magnetically labelled cells.

Downstream applications

T cells isolated with the Pan T Cell Isolation Kit can be used when direct labeling of T cell surface molecules could interfere with downstream applications, e.g., in studies on T cell activation.

^{1, 2}

T cells isolated with the Pan T Cell Isolation Kit have also been used to generate antigen-specific T cell lines.

^{3, 4}

Untouched T cells may also be used for further isolation of specific T cell subsets, such as naive

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or memory T cells and activated T cells.

Columns

LS or autoMACS

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Columns.

Figure 1

Separation of untouched T cells from PBMCs by depletion of non-T cells using the Pan T Cell Isolation Kit, an LS Column, and a MidiMACS™ Separator. Cells were labeled with CD2-PE and CD3-FITC and analyzed by flow cytometry using the MACSQuant® Analyzer.

PBMCs before separation	Untouched T cells

Figure 2
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Figure 2

Isolation of untouched T cells.

Isolation of untouched T cells.

Selected references
1. Wagner, M. et al. (2004) IL-12p70-dependent Th1 induction by human B cells requires combined activation with CD40 ligand and CpG DNA. J. Immunol. 172: 954-963
2. Finney, H. M. et al. (2004) Activation of resting human primary T cells with chimeric receptors: costimulation from CD28, inducible costimulator, CD134, and CD137 in series with signals from the TCR zeta chain. J. Immunol. 172: 104-113
3. Faure, S. et al. (2004) ERM proteins regulate cytoskeleton relaxation promoting T cell-APC conjugation. Nat. Immunol. 5: 272-279
4. Bukczynski, J. et al. (2004) Costimulatory ligand 4-1BBL (CD137L) as an efficient adjuvant for human antiviral cytotoxic T cell responses. Proc. Natl. Acad. Sci. U.S.A. 101: 1291-1296
5. Hafsi, N. et al. (2004)
  
  Human dendritic cells respond to
  Helicobacter pylori
  , promoting NK cell and Tʜ1-effector responses
  in vitro
  .
  
  J. Immunol. 173: 1249-1257
Brochures and posters

MACS Cell Pre-Enrichment for flow sorting
Customer reports and application notes

Generation of functional CAR T cells from pan T cells isolated by MACS
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technology

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Pan T Cell Isolation Kit, human

For research use only

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Detailed separation procedure

Downstream applications

Columns

Figure 1

Figure 2

Figure 2

Selected references

Brochures and posters

Customer reports and application notes

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