YILIMART - CD4 T Cell Isolation Kit, human

The CD4

⁺

T Cell Isolation Kit has been developed for fast isolation of untouched CD4

⁺

T helper cells from human peripheral blood mononuclear cells (PBMCs). This updated kit offers even better performance and a significantly shorter protocol and replaces the previous kit (#130-091-155).

Detailed separation procedure

Non-CD4

⁺

cells, i.e., CD8

⁺

T cells, monocytes, neutrophils, eosinophils, B cells, dendritic cells, NK cells, granulocytes, γ/δ T cells, or erythroid cells are labeled by using a cocktail of biotin-conjugated antibodies. The cocktail contains antibodies against CD8, CD14, CD15, CD16, CD19, CD36, CD56, CD123, TCR γ/δ, and CD235a (Glycophorin A).

Subsequently, non-target cells are magnetically labelled with the CD4

⁺

T Cell MicroBead Cocktail. Isolation of highly pure T cells is achieved by depletion of magnetically labelled cells.

Downstream applications

T helper cells isolated with the CD4

⁺

T Cell Isolation Kit can be used when direct labeling of CD4

⁺

T cell surface molecules could interfere with downstream applications, e.g., in studies on T helper cell activation.

^1–5

Untouched CD4

⁺

T cells may also be used for the isolation of specific T helper cell subsets, such as naive

⁶

or memory CD4

⁺

T cells and activated CD4

⁺

T cells.

Columns

LS or autoMACS

^®

Columns.

Figure 1

Isolation of untouched CD4

⁺

T cells from PBMCs using the CD4

⁺

T Cell Isolation Kit, a LS Column, and a MidiMACS™ Separator. Cells were labeled with CD4-PE and CD3-FITC and analyzed by flow cytometry using the MACSQuant® Analyzer.

PBMCs before separation	Untouched CD4 ⁺ T helper cells

Selected references
1. Zehn et al. (2004) Extended presentation of specific MHC-peptide complexes by mature dendritic cells compared to other types of antigen-presenting cells. Eur. J. Immunol. 34: 1551-1560
2. Wagner, M. et al. (2004) IL-12p70-dependent Th1 induction by human B cells requires combined activation with CD40 ligand and CpG DNA. J. Immunol. 172: 954-963
3. Crawford, G. E. et al. (2004) Identifying gene regulatory elements by genome-wide recovery of DNase hypersensitive sites. Proc. Natl. Acad. Sci. U.S.A. 101: 992-997
4. Finney, H. M. et al. (2004) Activation of resting human primary T cells with chimeric receptors: costimulation from CD28, inducible costimulator, CD134, and CD137 in series with signals from the TCR zeta chain. J. Immunol. 172: 104-113
5. Zitt, C. et al. (2004)
  
  Potent inhibition of Ca
  ²⁺
  release-activated Ca
  ²⁺
  channels and T-lymphocyte activation by the pyrazole derivative BTP2.
  
  J. Biol. Chem. 279: 12427-12437
6. Verhasselt et al. (2004) Induction of FOXP3-expressing regulatory CD4pos T cells by human mature autologous dendritic cells. Eur. J. Immunol. 34: 762-772
Brochures and posters

MACS Cell Pre-Enrichment for flow sorting

All you need for your T cell research
Special protocols

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⁺
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⁺
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⁺
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⁺
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CD4 T Cell Isolation Kit, human

For research use only

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Detailed separation procedure

Downstream applications

Columns

Figure 1

Selected references

Brochures and posters

Special protocols

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