YILIMART - CD8 T Cell Isolation Kit, human

The CD8

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T Cell Isolation Kit has been developed for fast isolation of untouched cytotoxic CD8

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T cells from human peripheral blood mononuclear cells (PBMCs). This updated kit offers even better performance and a significantly shorter protocol and replaces the previous kit (#130-094-156).

Detailed separation procedure

Non-CD8

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cells, i.e., CD4

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T cells, monocytes, neutrophils, eosinophils, B cells, stem cells, dendritic cells, NK cells, granulocytes, γ/δ T cells, or erythroid cells are labeled by using a cocktail of biotin-conjugated antibodies. The cocktail contains antibodies against CD4, CD15, CD16, CD19, CD34, CD36, CD56, CD123, TCRγ/δ, and CD235a (Glycophorin A). Subsequently, non-target cells are magnetically labelled with the CD8

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T Cell MicroBead Cocktail. Isolation of highly pure T cells is achieved by depletion of magnetically labelled cells.

Downstream applications

The CD8

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T Cell Isolation Kit is the tool of choice when direct labeling of CD8

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T cell surface molecules could interfere with downstream applications, e.g., in studies on cytotoxic T cell activation

¹

. Untouched CD8

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T cells may also be used for the isolation of specific cytotoxic T cell subsets, such as naive or memory CD8

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T cells and activated CD8

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T cells.

Columns

LS or autoMACS

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Columns.

Figure 1

Untouched CD8

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T cells were isolated from human PBMCs using the CD8

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T Cell Isolation Kit, an LS Column, and a MidiMACS™ Separator. Cells were fluorescently stained with CD8-FITC, CD56-PE, and CD3-APC to visualize the target cell fraction. Isolated CD8

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T cells were CD3

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and CD56

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. Cells were analyzed by flow cytometry using the MACSQuant

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Analyzer.

Unseparated fraction