Background information

Columns

• Figure 1

  GLAST + cells (positive fraction) were isolated from day 7 postnatal mouse brain tissue using the Neural Tissue Dissociation Kit (T), the gentleMACS™ Dissociator, FcR Blocking Reagent, mouse, the Anti-GLAST (ACSA-1) MicroBead Kit, a MiniMACS™ Separator, and an MS Column. Cells were fluorescently stained with Anti-GLAST (ACSA-1)-APC and analyzed by flow cytometry using the MACSQuant® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

  Mouse forebrain cells before separation	GLAST (ACSA-1)-negative cells
  GLAST (ACSA-1) -positive cells

• Selected references

  1. Storck, T. et al. (1992)
     Structure, expression, and functional analysis of a Na

     ⁺

     -dependent glutamate/aspartate transporter from rat brain.
     Proc. Natl. Acad. Sci. U.S.A. 8(22): 10955-10959
  2. Wahle, S. and Stoffel, W. (1996) Membrane topology of the high-affinity L-glutamate transporter (GLAST- 1) of the central nervous system. J. Cell Biol. 135(6): 1867-1877
  3. Kimelberg, H.K. (2004) The problem of astrocyte identity. Neurochem. Int. 45(2-3): 191-202
  4. Kriegstein, A. R. and Götz, M. (2003) Radial glia diversity: a matter of cell fate. Glia 43: 37-43

• Brochures and posters

  Neuroscience research
  Anti-GLAST (ACSA-1) products