YILIMART - CD127 Microbead Kit, human

The CD127 MicroBead Kit has been developed for the positive selection or depletion of CD127

⁺

cells from human PBMCs.

Background information

CD127, the α-chain of the IL-7 receptor, is a type I membrane glycoprotein. Signaling of IL-7 through the IL-7R requires both IL-7Rα and the common cytokine gamma chain (γc).

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CD127 can be identified on immature B cells through the early pre-B stage, on thymocytes, and on most mature T cells with transient down-regulation upon activation.

^2,3

On regulatory T cells CD127 is absent

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and its expression is inversely correlated with FoxP3 expression and suppressive function.

^5,6

CD127 is also used by thymic stromal derived lymphopoietin (TSLP) as part of a complex.

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Human regulatory T cells can be isolated, for example, by using the CD4

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CD25

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CD127

^dim/–

Regulatory T Cell Isolation Kit, human.

Columns

For positive selection: MS, LS, XS, or autoMACS

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Columns. For depletion: LD, D, or autoMACS Columns.

Figure 1

CD127

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cells were isolated from human PBMCs using the CD127 MicroBead Kit, an MS Column, and a MiniMACS™ Separator. Cells were fluorescently stained with CD4 (VIT4)-FITC and CD127-PE and analyzed by using the MACSQuant® Analyzer.

Unseparated fraction	Negative fraction

Isolated CD127 ⁺ cells

Selected references
1. Fry, T. J. and Mackall, C. L. (2002) Interleukin-7: from bench to clinic. Blood 99: 3892-3904
2. Sudo, T. et al. (1993) Expression and function of the interleukin 7 receptor in murine lymphocytes. Proc. Natl. Acad. Sci. U.S.A. 90: 9125-9129
3. Armitage, R. J. et al. (1991) Expression of receptors for interleukin 4 and interleukin 7 on human T cells. Adv. Exp. Med. Biol. 292: 121-130
4. Cupedo, T. et al. (2005) Development and activation of regulatory T cells in the human fetus. Eur. J. Immunol. 35: 383-390
5. Seddiki, N. et al. (2006) Expression of interleukin (IL)-2 and IL-7 receptors discriminates between human regulatory and activated T cells. J. Exp. Med. 203: 1693-1700
6. Liu, W. et al. (2006)
  
  CD127 expression inversely correlates with FoxP3 and suppressive function of human CD4
  ⁺
  T reg cells.
  
  J. Exp. Med. 203: 1701-1711

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CD127 Microbead Kit, human

For research use only

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Background information

Columns

Figure 1

Selected references

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