YILIMART - Diamond CD133 HSC Isolation Kit, human

The Diamond CD133 Isolation Kit was specifically developed for the isolation of highly pure Lin

^–

CD133

⁺

stem cells from peripheral blood, cord blood, bone marrow, and leukapheresis product by sequential sorting.

Detailed separation procedure

The Diamond CD133 MicroBead Kit includes a lineage depletion followed by a CD133 isolation. In the first step, cells are labeled with a cocktail of biotin-conjugated antibodies against lineage-specific antigens. These cells are subsequently labeled with Anti-Biotin MicroBeads. Lineage-negative stem and progenitor cells are obtained by depletion of the magnetically labeled cells. Then CD133

⁺

cells are isolated from the lineage-negative stem and progenitor cells using CD133 MicroBeads. The resulting population obtained are Lin

^-

CD133

⁺

stem cells.

Diamond CD133 MicroBeads recognize the epitope CD133/1. For control staining of separated cells, the application of CD133/2 (293C3) antibody is recommended.

Downstream applications

Lin

^-

CD133

⁺

stem cells can be used for many applications, including:

gene or miRNA expression profiling
characterization of undifferentiated stem cells and their developmental potential
stimulation of proliferation and maturation by cytokines
HSC-CFU assays
adoptive transfer and hematopoietic reconstitution studies

Columns

For the first magnetic separation (depletion): LD or autoMACS

^®

Columns. For the second magnetic separation (positive selection): MS or autoMACS Columns.

Figure 1

CD133

⁺

Lin

^–

cells were isolated from human PBMCs using the Diamond CD133 Isolation Kit, an LD Column, two MS Columns, a MidiMACS™ Separator, and a MiniMACS™ Separator. Cells were fluorescently stained with CD34-FITC, CD133/2 (293C3)-PE, CD45-APC, and Anti-Biotin-PE and analyzed using the MACSQuant® Analyzer.

PBMCs before separation	Lineage-negative fraction

Isolated CD133 ⁺ Lin ^- cells

Selected references
1. Estrada-González, P.K. et al. (2014) Decreased frequency, but normal functional integrity of mesenchymal stromal cells derived from untreated and Imatinib-treated chronic myeloid leukemia patients. Leuk. Res. 5: 594-600

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Diamond CD133 HSC Isolation Kit, human

For research use only

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Detailed separation procedure

Downstream applications

Columns

Figure 1

Selected references

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