Background information

Detailed separation procedure

Downstream applications

Columns

• Figure 1

  Myeloid-derived suppressor cells were isolated from a spleen of a BALB/c mouse using the Myeloid-Derived Suppressor Cell Isolation Kit, two LS Columns, two MS Columns, and an appropriate MACS Separator. Cells were fluorescently stained with CD11b-APC and Anti-Gr-1-PE and analyzed by flow cytometry. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

  Spleen cells before separation	Enriched Gr-1 high Ly-6G + cells
  Enriched Gr-1 dim Ly-6G – cells

• Selected references

  1. Serafini, R. et al. (2004) Derangement of immune responses by myeloid suppressor cells. Cancer Immunol. Immunother. 53: 64-72
  2. Youn, J. et al. (2008) Subsets of myeloid-derived suppressor cells in tumor-bearing mice. J. Immunol. 181: 5791-5802
  3. Ostrand-Rosenberg, S. and Sinha, P. (2009) Myeloid-derived suppressor cells: linking inflammation and cancer. J. Immunol. 182: 4499-4506
  4. Gabrilovich, D. I. and Nagaraj, S. (2009) Myeloid-derived suppressor cells as regulators of the immune system. Nat. Rev. Immunol. 9: 162-174
  5. Bronte, V. et al. (1999)
     Unopposed production of granulocyte-macrophage colony-stimulating factor by tumors inhibits CD8

     ⁺

     T cell responses by dysregulating antigen-presenting cell maturation.
     J. Immunol. 162: 5728-5737
  6. Movahedi, K. et al. (2008) Identification of discrete tumor-induced myeloid-derived suppressor cell subpopulations with distinct T cell-suppressive activity. Blood 111: 4233-4244

• Brochures and posters

  Isolation and analysis of murine myeloid cells from lymphoid tissues and tumor