YILIMART - CD8 CD45RA Effector T Cell Iso Kit, h

The CD8

⁺

CD45RA

⁺

Effector T Cell Isolation Kit has been developed for the positive selection of CD8

⁺

CD45RA

⁺

effector T cells from human PBMCs.

Background information

The CD8

⁺

CD45RA

⁺

Effector T Cell Isolation Kit is ideal for the isolation of cytotoxic T cells that express high levels of perforin and granzyme B

¹

. Isolated cells can be analyzed for interactions with other cell types and degranulation or secretion of effector molecules.

Detailed separation procedure

The isolation of CD8

⁺

CD45RA

⁺

effector T cells is performed in a two-step procedure. First, non-target cells are indirectly magnetically labeled for depletion with biotin-conjugated antibodies against CD45RO and CD56 and an APC-conjugated antibody against CD197 (CCR7), as well as Anti-APC MicroBeads and Anti-Biotin MicroBeads. In the second step, the CD8

⁺

CD45RA

⁺

effector T cells are directly labeled with CD8 MicroBeads and isolated by positive selection from the pre-enriched cell fraction.

Columns

For positive selection: MS, LS, or autoMACS

^®

Columns. For depletion: LD or autoMACS Columns.

Figure 1

CD8

⁺

CD45RA

⁺

effector T cells were isolated from human PBMCs using the CD8

⁺

CD45RA

⁺

Effector T Cell Isolation Kit, an LD Column, an MS Column, a MidiMACS™ Separator, and a MiniMACS™ Separator.

PBMCs before separation

Gated on viable CD8 ⁺ CD56 ^– cells.

Pre-enriched CD8 ⁺ CD45RA ⁺ effector T cells

Gated on viable CD8 ⁺ CD56 ^– cells.

Isolated CD8 ⁺ CD45RA ⁺ effector T cells

Gated on viable CD8 ⁺ CD56 ^– cells.

Selected references
1. Hamann, D. et al. (1997)
  
  Phenotypic and functional separation of memory and effector human CD8
  ⁺
  T cells.
  
  J. Exp. Med. 186: 1407-1418

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CD8 CD45RA Effector T Cell Iso Kit, h

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Background information

Detailed separation procedure

Columns

Figure 1

Selected references

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