Background information

Detailed separation procedure

Columns

• Figure 1

  Isolation of CD4 + RTEs from PBMCs using the CD4 + Recent Thymic Emigrant Isolation Kit, an LS Column, and an MS Column. To evaluate the isolation of CD4 + RTEs, PBMCs before separation and the isolated CD4 + RTEs were fluorescently stained with CD4, CD45RA, and CD31 antibodies and analyzed by flow cytometry.

  PBMCs before separation	PBMCs before separation gated on CD4 + CD45RA + cells
  Isolated CD4 + RTEs	CD4 + RTEs gated on CD4 + CD45RA + cells

• Selected references

  1. Kimmig, S. et al. (2002) Two subsets of naive T helper cells with distinct T cell receptor excision circle content in human adult peripheral blood. J. Exp. Med. 195: 789-794