YILIMART - Naive CD4 T Cell Iso Kit II, human

The Naive CD4

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T Cell Isolation Kit II was developed for the isolation of untouched naive CD4

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T cells from human peripheral blood.

Detailed separation procedure

For magnetic depletion of memory CD4

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T cells and non-CD4

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T cells, PBMCs are incubated with a cocktail of biotinylated CD45RO, CD8, CD14, CD15, CD16, CD19, CD25, CD34, CD36, CD56, CD123, anti-TCRγ/δ, anti-HLA-DR, and CD235a (glycophorin A) antibodies. These cells are subsequently magnetically labeled with Anti-Biotin MicroBeads for depletion.

Downstream applications

Naive CD4

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T cells isolated with the Naive CD4

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T Cell Isolation Kit II can be used when direct labeling of cell surface molecules could interfere with downstream applications, e.g., in studies on naive T helper cell activation and in functional studies of DCs.

^{1, 2}

Untouched naive CD4

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T cells can also be used for further isolation of specific T helper cell subsets.

For isolation of naive CD4

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recent thymic emigrants the CD4

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Recent Thymic Emigrant Isolation Kit, human is recommended.

Columns

LS, XS, or autoMACS

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Columns.

Figure 1

Isolation of untouched naive CD4

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T helper cells from PBMCs using the Naive CD4

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T Cell Isolation Kit II, an LS Column, and a MidiMACS™ Separator. Cells were stained with CD4 and CD45RA antibodies.

Unseparated fraction	Isolated untouched naive CD4 ⁺ T cells

Selected references
1. Shen, W. et al. (2005)
  
  Modulation of CD4 Tʜ cell differentiation by ganglioside GD1a
  in vitro
  .
  
  J. Immunol. 175: 4927-4934
2. Fernandez-Sesma, A. et al. (2006) Influenza virus evades innate and adaptive immunity via the NS1 protein. J. Virol. 80: 6295-6304
Customer reports and application notes

Effective and reliable in vitro generation of human Th17 cells. Hiller, J. et al. (2012)

MACS
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Technology facilitates mixed lymphocyte reaction

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Naive CD4 T Cell Iso Kit II, human

For research use only

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Detailed separation procedure

Downstream applications

Columns

Figure 1

Selected references

Customer reports and application notes

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