Background information

Downstream applications

Columns

• Figure 1

  Whole-brain tissue from P1 mice was first dissociated to single cells using the Neural Tissue Dissociation Kit (T) and then microglia were isolated using CD11b (Microglia) MicroBeads, an MS Column, and a MiniMACS™ Separator. Cells are fluorescently stained with CD11b-APC and CD45-FITC.

  Before separation	Microglia CD11b + fraction

• Selected references

  1. Justin, A. et al. (2012) The Ifng gene is essential for Vdr gene expression and vitamin D3-mediated reduction of the pathogenic T cell burden in the central nervous system in experimental autoimmune encephalomyelitis, a multiple sclerosis model. J. Immunol. 189: 3188-3197
  2. Lauren, L. et al. (2011) Microglia and memory: modulation by early-life infection. J. Neurosci. 31: 15511-15521
  3. Kremer, M. et al. (2010) Reduced immunoproteasome formation and accumulation of immunoproteasomal precursors in the brains of lymphocytic choriomeningitis virus-Infected mice. J. Immunol. 185: 5549-5560
  4. Lee, J. K. et al. (2008) Regulator of G-protein signaling 10 promotes dopaminergic neuron survival via regulation of the microglial inflammatory response. J. Neurosci. 28: 8517-8528
  5. Su, W. et al. (2014) The p53 transcription factor modulates microglia behavior through microRNA-dependent regulation of c-maf. J. Immunol. 192: 358-366
  6. Zhang, X. et al. (2014) Peripheral role of cathepsin S in Tʜ1 cell-dependent transition of nerve injury-induced acute pain to a chronic pain state. J. Neurosci. 34: 3013-3022
  7. Woodling, N. S. et al. (2014) Suppression of alzheimer-associated inflammation by microglial prostaglandin-E2 EP4 receptor signaling. J. Neurosci. 34: 5882-5894
  8. Kleinberger, G. et al. (2014) TREM2 mutations implicated in neurodegeneration impair cell surface transport and phagocytosis. Sci Transl Med 6: 243ra86
  9. Cho, S.-H. et al. (2015) SIRT1 deficiency in microglia contributes to cognitive decline in aging and neurodegeneration via epigenetic regulation of IL-1β. J. Neurosci. 35: 807-818
  10. Zeiner, P. S. et al. (2014) MIF receptor CD74 is restricted to microglia/macrophages, associated with a M1-polarized immune milieu and prolonged patient survival in gliomas. Brain Pathol. 25: 491-504
  11. Kiris, E. et al. (2015) Dysregulation of glutamine transporter SNAT1 in rett syndrome microglia: a mechanism for mitochondrial dysfunction and neurotoxicity. J. Neurosci. 35: 2516-2529

• Brochures and posters

  Neuroscience research

• Scientific posters

  Efficient isolation of viable primary neural cells from adult murine brain tissue based on a novel automated tissue dissociation protocol. Zhang, H. et al. SfN (2016)
  Semi-automated tissue dissociation and preserved epitope integrity optimize immunomagnetic sorting of neural cells

• Special protocols

  Isolation of microglia from adult mouse or rat brain