Background information

Detailed separation procedure

Downstream applications

Columns

• Figure 1

  CD4 + CD25 + CD45RA + regulatory T cells were isolated from human PBMCs by using the CD4 + CD25 + CD45RA + Regulatory T Cell Isolation Kit, an LD, and two MS Columns, a MidiMACS™ Separator, and a MiniMACS™ Separator. The isolated cells were fluorescently stained with CD4, CD25, and either CD45RA or Anti-FoxP3 antibodies. Dot plots show data gated on viable lymphocytes (C) or viable CD4 + lymphocytes (A, B).

  Viable CD4 + lymphocytes

  Viable lymphocytes

• Selected references

  1. Hoffmann, P. et al. (2006)
     Only the CD45RA

     ⁺

     subpopulation of CD4

     ⁺

     CD25

     ^high

     T cells gives rise to homogeneous regulatory T-cell lines upon

     in vitro

     expansion.
     Blood 108: 4260-4267

• Brochures and posters

  Regulatory T cell research