YILIMART - CD10 MicroBead Kit, human

The CD10 MicroBead Kit was developed for the enrichment or depletion of CD10

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cells from bone marrow or other cell sources.

Background information

CD10, the human common acute lymphoblastic leukemia antigen (CALLA), is a 100 kDa cell surface molecule identical to human membrane-associated neutral endopeptidase (NEP) and also known as neprilysin or enkephalinase. Human CD10 is expressed on a wide variety of normal and neoplastic cell types from different tissues including neural and hematopoietic cells.

CD10 is expressed on pre– and pro–B cells and is involved in B cell development and differentiation. The antigen is also present on mature neutrophils, T cell precursors, some T cell leukemias/lymphomas and on neoplastic cells of several B cell leukemias/lymphomas.¹

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Applications

The CD10 MicroBead Kit was developed for the enrichment or depletion of CD10

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cells from bone marrow or other cell sources. It can also be used for the isolation of CD10

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B cells from B cell leukemias/lymphomas.

Columns

For positive selection: MS, LS, XS, or autoMACS

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Columns. For depletion: LD, D, or autoMACS Columns.

Figure 1

CD10

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cells were isolated from human PBMCs spiked with Raji Burkitt´s lymphoma cells using the CD10 MicroBead Kit, an MS Column, and a MiniMACS™ Separator. Cells were fluorescently stained with Anti-Biotin-APC and CD19-PE and analyzed by flow cytometry.

Unseparated fraction	After separation

Selected references
1. Shipp, M. A. and Look, A. T. (1993) Hematopoietic differentiation antigens that are membrane-associated enzymes: cutting is the key! Blood 82: 1052-1070
2. Kalled, S. L. et al. (1995) The distribution of CD10 (NEP 24.11, CALLA) in human and mice is similar in non-lymphoid organs but differ within the hematopoietic system: absence on murine T and B lymphoid progenitors. Eur. J. Immunol. 25: 677-687
Special protocols

Isolation of CD10
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B cells from human bone marrow or lysed blood

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CD10 MicroBead Kit, human

For research use only

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Background information

Applications

Columns

Figure 1

Selected references

Special protocols

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