YILIMART - CD25 MicroBeads II, human

CD25 MicroBeads II, human

For research use only

货号：: 130-092-983
描述：: CD25 MicroBeads II, human
包装：: for 1×10⁹ total cells

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CD25 MicroBeads II were developed for positive selection or depletion of CD25

⁺

cells.

Background information

CD25, the low-affinity interleukin-2 receptor alpha chain (IL-2Rα), is expressed on activated T and B cells and monocytes. CD25 is also present on a subset of non-activated CD4

⁺

T cells, which has regulatory function.

The CD25 MicroBeads II recognize epitope A of the CD25 molecule.

For control staining of cells separated with CD25 MicroBeads II, it is recommended to use MACS

^®

CD25 Antibodies, which bind to epitope B of the CD25 antigen.

For isolation of CD25

⁺

cell subsets, the CD25 MicroBeads II can be combined with MACS

^®

Cell Isolation Kits or MultiSort Kits. The CD4

⁺

CD25

⁺

Regulatory T Cell Isolation Kit is recommended for most efficient separation of CD4

⁺

CD25

⁺

regulatory T cells.

Columns

For positive selection: MS, LS, XS, or autoMACS

^®

Columns. For depletion: LD, D, or autoMACS Columns.

Figure 1

Depletion of CD25

⁺

cells from PBMCs using CD25 MicroBeads II, an LD Column, and a MidiMACS™ Separator.

PBMCs before separation	CD25 ⁺ cell depleted fraction