YILIMART - Anti-PSA-NCAM MicroBeads,HMR

Anti-PSA-NCAM MicroBeads have been developed for the positive selection or depletion of PSA-NCAM

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cells from tissue or cell culture.

Background information

Anti-PSA-NCAM MicroBeads recognize polysialic acid (PSA), which is linked to the extracellular domain of the neural cell adhesion molecule (NCAM, CD56).

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PSA-NCAM, the highly polysialated form of NCAM, is predominantly expressed in embryonic and neonatal neural tissue

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. In adult mammalian brain PSA-NCAM expression is restricted mainly to areas that retain neurogenic potential, such as the subventricular zone (SVZ)

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and the dentate gyrus of the hippocampus.

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PSA-NCAM is a marker for immature neuronal-committed progenitors that are permanently generated in the SVZ and migrate along a well-defined pathway, the rostral migratory stream, into the olfactory bulb where they differentiate into GABAergic and dopaminergic interneurons.

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PSA-NCAM positive neuronal precursors have been isolated from rat forebrain and from mouse SVZ tissue after depletion of A2B5

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glial progenitor cells.

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Columns

For positive selection: MS, LS, or autoMACS

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Columns. For depletion: LD or autoMACS Columns.

Figure 1

Separation of a single-cell suspension derived from P1 mouse whole-brain tissue using the Neural Tissue Dissociation Kit (T), Anti-PSA-NCAM MicroBeads, a MiniMACS™ Separator, and an MS Column. Cells were fluorescently stained with rat anti-mouse IgM-APC and analyzed by flow cytometry.

A:	B:
Neural cells before separation	PSA-NCAM ^- cells

C:
PSA-NCAM ⁺ cells

Selected references
1. Rougon, G. and Marshak, D. R. (1986) Structural and immunological characterization of the amino-terminal domain of mammalian neural cell adhesion molecules. J. Biol. Chem. 261: 3396-3401
2. Kiss, J. Z. and Muller, D. (2001) Contribution of the neural cell adhesion molecule to neuronal and synaptic plasticity. Rev. Neurosci. 12: 297-310
3. Doetsch, F. et al. (1997) Cellular composition and three-dimensional organization of the subventricular germinal zone in the adult mammalian brain. J. Neurosci. 17: 5046-5061
4. Seki, T. (2002) Expression patterns of immature neuronal markers PSA-NCAM, CRMP-4 and NeuroD in the hippocampus of young adult and aged rodents. J. Neurosci. Res. 70: 327-334
5. Pennartz, S. et al. (2004) Purification of neuronal precursors from the adult mouse brain: comprehensive gene expression analysis provides new insights into the control of cell migration, differentiation, and homeostasis. Mol. Cell. Neurosci. 25: 692-706
6. Seidenfaden, R. et al. (2006) Glial conversion of SVZ-derived committed neuronal precursors after ectopic grafting into the adult brain. Mol. Cell. Neurosci. 32: 187-198
7. Seidenfaden et al. (2006) MACS&more 10(1): 4-6
8. Marmur, R. et al. (1998) Differentiation of oligodendroglial progenitors derived from cortical multipotent cells requires extrinsic signals including activation of gp130/LIFbeta receptors. J. Neurosci. 18: 9800-9811
9. Strathmann et al. (2007) Identification of two novel glial-restricted cell populations in the embryonic telencephalon arising from unique origins. BMC Dev. Biol. 7: 33
Scientific posters

Semi-automated tissue dissociation and preserved epitope integrity optimize immunomagnetic sorting of neural cells
Customer reports and application notes

Purification of neuronal precursors. Seidenfaden et al. (2010)

Intracellular labeling of multiple cell types for MRI-based in vivo cell tracking. Kernbach et al. (2012) MACS&more 14(2).

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Anti-PSA-NCAM MicroBeads,HMR

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Background information

Columns

Figure 1

Selected references

Scientific posters

Customer reports and application notes

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