Background information

Detailed separation procedure

Downstream applications

Columns

• Figure 1

  Isolation of CD105 + Sca-1 + LTR-HSCs from mouse bone marrow using the CD105 MultiSort Kit (PE), mouse, and two LS Columns for the first positive selection followed by the Anti-Sca-1 MicroBead Kit (FITC), mouse, and two MS Columns in the second positive selection step.

  A:	B:
  Unseparated fraction	CD105 + cells
  C:
  CD105+ Sca-1+ LTR-HSCs

• Selected references

  1. Chen, C. Z. et al. (2002) Identification of endoglin as a functional marker that defines long-term repopulating hematopoietic stem cells. Proc. Natl. Acad. Sci. U.S.A. 99: 15468-15473
  2. Chen, C. Z. et al. (2003) The endoglin(positive) sca-1(positive) rhodamine(low) phenotype defines a near-homogeneous population of long-term repopulating hematopoietic stem cells. Immunity 19: 525-533

• Special protocols

  Isolation of CD105

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  Sca-1

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  LTR-HSCs from mouse bone marrow