YILIMART - CD209 (DC-SIGN) MB Kit, human

The CD209 (DC-SIGN) MicroBead Kit has been developed for the magnetic separation of human CD209

⁺

cells.

Background information

CD209 (Dendritic Cell Specific ICAM-3 Grabbing Non-integrin, DC-SIGN) is a type II C-type lectin receptor. It is expressed on DCs in dermal and mucous tissue, in lymphoid tissue such as tonsil, lymph node, and spleen, and on monocyte-derived DCs (Mo-DCs). It is not expressed on DC subsets in peripheral blood, except for a subpopulation of CD14

⁺

cells with DC-like phenotype. DC-SIGN acts as an adhesion receptor to facilitate interactions between DCs and T cells or DCs and endothelial cells. In addition, it serves as an antigen receptor to mediate internalization of ligands for antigen presentation, binding viruses such as HIV, HCMV, or Ebola as well as parasites, bacteria, or yeast.

Applications

The CD209 (DC-SIGN) MicroBead Kit can be used for positive selection or depletion of cells expressing the human CD209 (DC-SIGN) antigen.

Columns

For positive selection: MS, LS, XS, or autoMACS

^®

Columns. For depletion: LD, D, or autoMACS Columns.

Figure 1

Separation of CD209

⁺

cells from human PBMCs spiked with 1% Mo-DCs using the CD209 MicroBead Kit, two MS Columns, and a MiniMACS™ Separator. Mo-DCs were generated in vitro with GM-CSF and IL-4 for 7 days. Cells were stained with CD209 (DC-SIGN)-FITC and CD304 (BDCA-4/Neuropilin-1)-PE and analyzed by flow cytometry.

Unseparated fraction	Cells depleted of

Isolated CD209 (DC-SIGN) ⁺ cells

Selected references
1. Koppel, E. A. et al. (2005) Distinct functions of DC-SIGN and its homologues L-SIGN (DC-SIGNR) and mSIGNR1 in pathogen recognition and immune regulation. Cell. Microbiol. 7: 157-165

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CD209 (DC-SIGN) MB Kit, human

For research use only

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Background information

Applications

Columns

Figure 1

Selected references

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